Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500009 |
Resumo: | PURPOSE: To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. METHODS: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. RESULTS: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). CONCLUSION: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones. |
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Acta Cirúrgica Brasileira (Online) |
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Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioningTransplantation, AutologousIschemic PreconditioningVaginal SmearsOvaryRatsPURPOSE: To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. METHODS: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. RESULTS: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). CONCLUSION: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2009-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500009Acta Cirúrgica Brasileira v.24 n.5 2009reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502009000500009info:eu-repo/semantics/openAccessDamous,Luciana LamarãoSilva,Sônia Maria daLopes,Renato Antônio MiglianoSakano,Célia Regina de Souza BezerraSimões,Manuel de JesusMontero,Edna Frasson de Souzaeng2009-10-16T00:00:00Zoai:scielo:S0102-86502009000500009Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2009-10-16T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
title |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
spellingShingle |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning Damous,Luciana Lamarão Transplantation, Autologous Ischemic Preconditioning Vaginal Smears Ovary Rats |
title_short |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
title_full |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
title_fullStr |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
title_full_unstemmed |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
title_sort |
Study on the vaginal smear of rats submitted to autologous ovarian transplant: impact of remote ischemic preconditioning |
author |
Damous,Luciana Lamarão |
author_facet |
Damous,Luciana Lamarão Silva,Sônia Maria da Lopes,Renato Antônio Migliano Sakano,Célia Regina de Souza Bezerra Simões,Manuel de Jesus Montero,Edna Frasson de Souza |
author_role |
author |
author2 |
Silva,Sônia Maria da Lopes,Renato Antônio Migliano Sakano,Célia Regina de Souza Bezerra Simões,Manuel de Jesus Montero,Edna Frasson de Souza |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Damous,Luciana Lamarão Silva,Sônia Maria da Lopes,Renato Antônio Migliano Sakano,Célia Regina de Souza Bezerra Simões,Manuel de Jesus Montero,Edna Frasson de Souza |
dc.subject.por.fl_str_mv |
Transplantation, Autologous Ischemic Preconditioning Vaginal Smears Ovary Rats |
topic |
Transplantation, Autologous Ischemic Preconditioning Vaginal Smears Ovary Rats |
description |
PURPOSE: To evaluate the remote ischemic preconditioning (R-IPC) impact on the quality of the ovarian graft by means of vaginal smear of transplanted rats. METHODS: Sixty rats were used divided in six groups: Control; Fresh transplant (TxF); Cryopreserved transplant (TxC); R-IPC; R-IPC + fresh transplant (TxF+R-IPC); R-IPC + cryopreserved transplant (TxC+R-IPC). R-IPC was performed in the common iliac artery. Autologous ovarian tissue was implanted integrally in the retro peritoneum. On the first PO day, vaginal smear collection was daily initiated. After 30 days, the PO day when the estrous cycle was re-initiated was considered for analysis as well as the estrous days and the number of estrous cycles. RESULTS: R-IPC showed a tendency to an early estrus re-initiation (p>0.05) as well as increase the number of cycles in the fresh transplanted group while in the cryopreserved transplant the number of cycles was similar, regardless of the stimulus R-IPC (p>0.05). The animals which had undergone fresh grafts had a longer estrous period than the ones which had undergone cryopreserved grafts, with or without R-IPC (p<0.05). CONCLUSION: R-IPC promoted earlier re-initiation of ovarian activity in the PO and greater estrous frequency, with more consistent results in the fresh grafts than in the cryopreserved ones. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502009000500009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-86502009000500009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.24 n.5 2009 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126439610646528 |