Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Detalhes bibliográficos
Autor(a) principal: Éboli,Lígia Patrícia de Carvalho Batista
Data de Publicação: 2016
Outros Autores: Netto,Alcides Augusto Salzedas, Azevedo,Ramiro Antero de, Lanzoni,Valéria Pereira, Paula,Tatiana Sugayama de, Goldenberg,Alberto, Gonzalez,Adriano Miziara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016001200783
Resumo: ABSTRACT PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) . RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.
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spelling Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamineLiver FailureAcuteGalactosamineModelsAnimalRatsABSTRACT PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) . RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016001200783Acta Cirúrgica Brasileira v.31 n.12 2016reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/s0102-865020160120000002info:eu-repo/semantics/openAccessÉboli,Lígia Patrícia de Carvalho BatistaNetto,Alcides Augusto SalzedasAzevedo,Ramiro Antero deLanzoni,Valéria PereiraPaula,Tatiana Sugayama deGoldenberg,AlbertoGonzalez,Adriano Miziaraeng2017-01-06T00:00:00Zoai:scielo:S0102-86502016001200783Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2017-01-06T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
title Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
spellingShingle Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
Éboli,Lígia Patrícia de Carvalho Batista
Liver Failure
Acute
Galactosamine
Models
Animal
Rats
title_short Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
title_full Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
title_fullStr Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
title_full_unstemmed Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
title_sort Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine
author Éboli,Lígia Patrícia de Carvalho Batista
author_facet Éboli,Lígia Patrícia de Carvalho Batista
Netto,Alcides Augusto Salzedas
Azevedo,Ramiro Antero de
Lanzoni,Valéria Pereira
Paula,Tatiana Sugayama de
Goldenberg,Alberto
Gonzalez,Adriano Miziara
author_role author
author2 Netto,Alcides Augusto Salzedas
Azevedo,Ramiro Antero de
Lanzoni,Valéria Pereira
Paula,Tatiana Sugayama de
Goldenberg,Alberto
Gonzalez,Adriano Miziara
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Éboli,Lígia Patrícia de Carvalho Batista
Netto,Alcides Augusto Salzedas
Azevedo,Ramiro Antero de
Lanzoni,Valéria Pereira
Paula,Tatiana Sugayama de
Goldenberg,Alberto
Gonzalez,Adriano Miziara
dc.subject.por.fl_str_mv Liver Failure
Acute
Galactosamine
Models
Animal
Rats
topic Liver Failure
Acute
Galactosamine
Models
Animal
Rats
description ABSTRACT PURPOSE: To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS: Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats) . RESULTS: Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION: King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.
publishDate 2016
dc.date.none.fl_str_mv 2016-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016001200783
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016001200783
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0102-865020160120000002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.31 n.12 2016
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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