Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats

Detalhes bibliográficos
Autor(a) principal: Freitas,André Luiz Pires de
Data de Publicação: 2014
Outros Autores: Silva,Marcelo de Oliveira e, Matsumoto,Priscilla Martin Keiko, Han,Sang Won, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400007
Resumo: PURPOSE:To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS:Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS:obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION:It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
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spelling Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in ratsStem CellsAdipose TissueAnimal ModelsMesenchymal Stem Cell TransplantationSurgical FlapsPURPOSE:To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS:Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS:obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION:It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400007Acta Cirúrgica Brasileira v.29 suppl.2 2014reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502014001400006info:eu-repo/semantics/openAccessFreitas,André Luiz Pires deSilva,Marcelo de Oliveira eMatsumoto,Priscilla Martin KeikoHan,Sang WonFerreira,Lydia Masakoeng2015-10-08T00:00:00Zoai:scielo:S0102-86502014001400007Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2015-10-08T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
spellingShingle Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
Freitas,André Luiz Pires de
Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
title_short Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_full Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_fullStr Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_full_unstemmed Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_sort Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
author Freitas,André Luiz Pires de
author_facet Freitas,André Luiz Pires de
Silva,Marcelo de Oliveira e
Matsumoto,Priscilla Martin Keiko
Han,Sang Won
Ferreira,Lydia Masako
author_role author
author2 Silva,Marcelo de Oliveira e
Matsumoto,Priscilla Martin Keiko
Han,Sang Won
Ferreira,Lydia Masako
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Freitas,André Luiz Pires de
Silva,Marcelo de Oliveira e
Matsumoto,Priscilla Martin Keiko
Han,Sang Won
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
topic Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
description PURPOSE:To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS:Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS:obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION:It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014001400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502014001400006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.29 suppl.2 2014
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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