Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats

Detalhes bibliográficos
Autor(a) principal: Freitas, André Luiz Pires de [UNIFESP]
Data de Publicação: 2014
Outros Autores: Silva, Marcelo de Oliveira e, Matsumoto, Priscilla Martin Keiko, Han, Sang Won [UNIFESP], Ferreira, Lydia Masako [UNIFESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNIFESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502014001400006
http://repositorio.unifesp.br/handle/11600/8146
Resumo: PURPOSE: To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS: Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS: obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION: It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
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spelling Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in ratsStem CellsAdipose TissueAnimal ModelsMesenchymal Stem Cell TransplantationSurgical FlapsPURPOSE: To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS: Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS: obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION: It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.Federal University of São PauloUNIFESPUNIFESPSciELOSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade Federal de São Paulo (UNIFESP)Freitas, André Luiz Pires de [UNIFESP]Silva, Marcelo de Oliveira eMatsumoto, Priscilla Martin KeikoHan, Sang Won [UNIFESP]Ferreira, Lydia Masako [UNIFESP]2015-06-14T13:46:49Z2015-06-14T13:46:49Z2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion29-33application/pdfhttp://dx.doi.org/10.1590/S0102-86502014001400006Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 29-33, 2014.10.1590/S0102-86502014001400006S0102-86502014001400007.pdf0102-8650S0102-86502014001400007http://repositorio.unifesp.br/handle/11600/8146WOS:000342065300007engActa Cirurgica Brasileirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-07-29T20:30:13Zoai:repositorio.unifesp.br/:11600/8146Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-07-29T20:30:13Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false
dc.title.none.fl_str_mv Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
spellingShingle Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
Freitas, André Luiz Pires de [UNIFESP]
Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
title_short Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_full Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_fullStr Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_full_unstemmed Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
title_sort Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats
author Freitas, André Luiz Pires de [UNIFESP]
author_facet Freitas, André Luiz Pires de [UNIFESP]
Silva, Marcelo de Oliveira e
Matsumoto, Priscilla Martin Keiko
Han, Sang Won [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
author_role author
author2 Silva, Marcelo de Oliveira e
Matsumoto, Priscilla Martin Keiko
Han, Sang Won [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Freitas, André Luiz Pires de [UNIFESP]
Silva, Marcelo de Oliveira e
Matsumoto, Priscilla Martin Keiko
Han, Sang Won [UNIFESP]
Ferreira, Lydia Masako [UNIFESP]
dc.subject.por.fl_str_mv Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
topic Stem Cells
Adipose Tissue
Animal Models
Mesenchymal Stem Cell Transplantation
Surgical Flaps
description PURPOSE: To investigate the experimental model for obtaining adipose tissue, isolation, characterization of mesenchymal stem cells and evaluation of their distribution in the tram flap in rats.METHODS: Five rats of Wistar were randomly assigned to two groups. In group I, three animals underwent removal of adipose tissue in the groin procedure to establish the experimental model and obtain a cell lineage. The animals of group II (n = 2) underwent surgical flap procedure, and satisfaction injection of mesenchymal stem cells pretreated with marker fluorescente.RESULTS: obtaining adipose tissue of the inguinal region of the rat proved to be possible. The isolated cells were characterized as mesenchymal stem cells and fluorescence microscopy showed the presence of multiple cells arranged around blood vessels and capillaries.CONCLUSION: It was possible to establish an experimental model for obtaining adipose tissue for isolation of mesenchymal stem cells and their distribution in the TRAM flap in rats.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
2015-06-14T13:46:49Z
2015-06-14T13:46:49Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502014001400006
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 29-33, 2014.
10.1590/S0102-86502014001400006
S0102-86502014001400007.pdf
0102-8650
S0102-86502014001400007
http://repositorio.unifesp.br/handle/11600/8146
WOS:000342065300007
url http://dx.doi.org/10.1590/S0102-86502014001400006
http://repositorio.unifesp.br/handle/11600/8146
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, p. 29-33, 2014.
10.1590/S0102-86502014001400006
S0102-86502014001400007.pdf
0102-8650
S0102-86502014001400007
WOS:000342065300007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 29-33
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv reponame:Repositório Institucional da UNIFESP
instname:Universidade Federal de São Paulo (UNIFESP)
instacron:UNIFESP
instname_str Universidade Federal de São Paulo (UNIFESP)
instacron_str UNIFESP
institution UNIFESP
reponame_str Repositório Institucional da UNIFESP
collection Repositório Institucional da UNIFESP
repository.name.fl_str_mv Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)
repository.mail.fl_str_mv biblioteca.csp@unifesp.br
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