Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats

Detalhes bibliográficos
Autor(a) principal: Brito,Nara Macedo Botelho
Data de Publicação: 2007
Outros Autores: Brito,Marcus Vinicius Henriques, Carvalho,Rita de Kássia Vidigal, Matos,Lia Tavares de Moura Brasil, Veloso,Thiago Sousa, Vasconcelos,Diego Matos de, Brito,Rosângela Baía
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000600014
Resumo: PURPOSE: To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. METHODS: Fifteen female rats were used, and assigned to three groups each one with five rats: group A - rats with 4x10(6) cells of Walker 256 carcinoma without acid acetic inoculation; group B - rats with 2x10(6) cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10(6) cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance - ANOVA. P values < 0.05 were taken to indicate statistical significance. RESULTS: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. CONCLUSION: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10(6) or 4x10(6) cells on its inoculation.
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spelling Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female ratsCarcinoma 256, WalkerCervix uteriVaginaDisease models, animalRatsPURPOSE: To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. METHODS: Fifteen female rats were used, and assigned to three groups each one with five rats: group A - rats with 4x10(6) cells of Walker 256 carcinoma without acid acetic inoculation; group B - rats with 2x10(6) cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10(6) cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance - ANOVA. P values < 0.05 were taken to indicate statistical significance. RESULTS: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. CONCLUSION: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10(6) or 4x10(6) cells on its inoculation.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000600014Acta Cirúrgica Brasileira v.22 n.6 2007reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-86502007000600014info:eu-repo/semantics/openAccessBrito,Nara Macedo BotelhoBrito,Marcus Vinicius HenriquesCarvalho,Rita de Kássia VidigalMatos,Lia Tavares de Moura BrasilVeloso,Thiago SousaVasconcelos,Diego Matos deBrito,Rosângela Baíaeng2008-01-22T00:00:00Zoai:scielo:S0102-86502007000600014Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2008-01-22T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
title Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
spellingShingle Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
Brito,Nara Macedo Botelho
Carcinoma 256, Walker
Cervix uteri
Vagina
Disease models, animal
Rats
title_short Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
title_full Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
title_fullStr Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
title_full_unstemmed Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
title_sort Experimental inoculation model of Walker 256 carcinoma into vagina and cervix uteri of female rats
author Brito,Nara Macedo Botelho
author_facet Brito,Nara Macedo Botelho
Brito,Marcus Vinicius Henriques
Carvalho,Rita de Kássia Vidigal
Matos,Lia Tavares de Moura Brasil
Veloso,Thiago Sousa
Vasconcelos,Diego Matos de
Brito,Rosângela Baía
author_role author
author2 Brito,Marcus Vinicius Henriques
Carvalho,Rita de Kássia Vidigal
Matos,Lia Tavares de Moura Brasil
Veloso,Thiago Sousa
Vasconcelos,Diego Matos de
Brito,Rosângela Baía
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Brito,Nara Macedo Botelho
Brito,Marcus Vinicius Henriques
Carvalho,Rita de Kássia Vidigal
Matos,Lia Tavares de Moura Brasil
Veloso,Thiago Sousa
Vasconcelos,Diego Matos de
Brito,Rosângela Baía
dc.subject.por.fl_str_mv Carcinoma 256, Walker
Cervix uteri
Vagina
Disease models, animal
Rats
topic Carcinoma 256, Walker
Cervix uteri
Vagina
Disease models, animal
Rats
description PURPOSE: To establish an inoculation model of Walker 256 carcinoma on cervix uteri and vagina of rats. METHODS: Fifteen female rats were used, and assigned to three groups each one with five rats: group A - rats with 4x10(6) cells of Walker 256 carcinoma without acid acetic inoculation; group B - rats with 2x10(6) cells of Walker 256 carcinoma with acid acetic inoculation and group C: rats with 4x10(6) cells of Walker 256 carcinoma with acid acetic inoculation. The day before tumor cells inoculation the rats from groups B and C were anaesthetized with diethylether and 0,3 ml of acetic acid was inoculated into their vaginas. Tumor cell inoculation into the vagina and cervix was done under general anesthesia with diethylether. Then a endocervical brush was used to scrape the vaginal wall and after that 0,3 ml of the liquid containing tumor cells was inoculated on the vagina and cervix. For the tumor analysis, animals were euthanized at day 12 following tumor cell implantation by an excessive inhalation of diethylether. Tumor was resected entirely and weighed and the tumors were then sectioned and counter stained with hematoxylin and eosin for histopathologic evaluation. It was also calculated the percentage of tumor equivalent to the body weight by the formula: P= tumor weight / body weight x 100. Data were analyzed by one-way analysis of variance - ANOVA. P values < 0.05 were taken to indicate statistical significance. RESULTS: Implantation and growth on GB and GC was 100% and on GA 20%. There was no statistical difference between GB and GC averages. CONCLUSION: According to the methods used, the Walker 256 carcinoma inoculation model into vagina and cervix have an implantation and growth rate of 100% when associated with previous acid acetic inoculation and there is no behavioral difference between using 2x10(6) or 4x10(6) cells on its inoculation.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000600014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502007000600014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-86502007000600014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.22 n.6 2007
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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