Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil

Detalhes bibliográficos
Autor(a) principal: Dianese,Érico de C.
Data de Publicação: 2005
Outros Autores: Ramalho,Eduardo D., Cerqueira,Daniela M., Lopes,Daniela B., Fajardo,Thor V.M., Ferreira,Marisa A.S.V., Martins,Cláudia R.F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Fitopatologia Brasileira
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012
Resumo: Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.
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spelling Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, BrazilGLRaV-3Vitis spp.AmpelovirusRNA dependent RNA polymeraseMany viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.Sociedade Brasileira de Fitopatologia2005-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012Fitopatologia Brasileira v.30 n.2 2005reponame:Fitopatologia Brasileirainstname:Sociedade Brasileira de Fitopatologia (SBF)instacron:SBF10.1590/S0100-41582005000200012info:eu-repo/semantics/openAccessDianese,Érico de C.Ramalho,Eduardo D.Cerqueira,Daniela M.Lopes,Daniela B.Fajardo,Thor V.M.Ferreira,Marisa A.S.V.Martins,Cláudia R.F.eng2005-05-17T00:00:00Zoai:scielo:S0100-41582005000200012Revistahttp://www.scielo.br/fbONGhttps://old.scielo.br/oai/scielo-oai.php||sbf-revista@ufla.br1678-46770100-4158opendoar:2005-05-17T00:00Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)false
dc.title.none.fl_str_mv Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
spellingShingle Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
Dianese,Érico de C.
GLRaV-3
Vitis spp.
Ampelovirus
RNA dependent RNA polymerase
title_short Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_full Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_fullStr Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_full_unstemmed Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
title_sort Variability of the 3' terminal of the polymerase gene of Grapevine leafroll-associated virus 3 isolates from Vale do São Francisco, Brazil
author Dianese,Érico de C.
author_facet Dianese,Érico de C.
Ramalho,Eduardo D.
Cerqueira,Daniela M.
Lopes,Daniela B.
Fajardo,Thor V.M.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
author_role author
author2 Ramalho,Eduardo D.
Cerqueira,Daniela M.
Lopes,Daniela B.
Fajardo,Thor V.M.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dianese,Érico de C.
Ramalho,Eduardo D.
Cerqueira,Daniela M.
Lopes,Daniela B.
Fajardo,Thor V.M.
Ferreira,Marisa A.S.V.
Martins,Cláudia R.F.
dc.subject.por.fl_str_mv GLRaV-3
Vitis spp.
Ampelovirus
RNA dependent RNA polymerase
topic GLRaV-3
Vitis spp.
Ampelovirus
RNA dependent RNA polymerase
description Many viral diseases, including leafroll, which is of great economic importance, affect grapevines (Vitis spp.). A complex of eight viruses [Grapevine leafroll-associated virus (GLRaV) -1 to 8] is associated with this disease. The objective of this study was to compare the variability of the 3' terminal region of the polymerase gene of three isolates of GLRaV-3 (Grapevine leafroll-associated virus-3), from Submédio do Vale do Rio São Francisco (Petrolina-PE) with that of other isolates available at the GenBank, including an isolate from North America and another from Southern Brazil. The viral RNA was extracted from three infected ELISA reactive plants and a fragment of 340 bp was amplified, by RT-PCR, using primers that recognize that portion of the polymerase gene found between nucleotides 8267 and 8606. The three isolates from Vale do Rio São Francisco named Pet-1, Pet-2 and Pet-3, showed similarities ranging from 98% and 94%, respectively to the isolates from North America (AF037268) and Southern Brazilian (AF438411). Considering the whole genome, the main variation found was one amino acid change at position 2766 (F2766Y). These preliminary data indicate the existence of a natural variation among GLRaV-3 isolates from grapevines. This could be due to the vegetative propagation and long cycle of the plant, associated with the error-prone nature of RNA-dependent RNA polymerase.
publishDate 2005
dc.date.none.fl_str_mv 2005-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000200012
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-41582005000200012
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
publisher.none.fl_str_mv Sociedade Brasileira de Fitopatologia
dc.source.none.fl_str_mv Fitopatologia Brasileira v.30 n.2 2005
reponame:Fitopatologia Brasileira
instname:Sociedade Brasileira de Fitopatologia (SBF)
instacron:SBF
instname_str Sociedade Brasileira de Fitopatologia (SBF)
instacron_str SBF
institution SBF
reponame_str Fitopatologia Brasileira
collection Fitopatologia Brasileira
repository.name.fl_str_mv Fitopatologia Brasileira - Sociedade Brasileira de Fitopatologia (SBF)
repository.mail.fl_str_mv ||sbf-revista@ufla.br
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