Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Tropical plant pathology (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200004 |
Resumo: | Commercial grapevine fruit (Vitis vinifera) of the Red Globe variety were collected in vineyards from Vale do São Francisco lower basin, an area of occurrence of grapevine bacterial canker. Seeds were extracted, classified as symptomatic or asymptomatic and processed in order to be observed under light (LM) and scanning electron microscopy (SEM) along with silver-enhanced immunogold labeling, to allow bacterial detection using a policlonal antibody against Xanthomonas campestris pv. viticola (Xcvi), etiological agent of the disease. The seed samples showed bacterial aggregates associated to the tegument surface and to the first parenchymal layer beneath the seed tegument. Bacterial identity was confirmed by immunogold labeling. This appears to be the first report of Xcvi associated to asymptomatic seeds and berries, suggesting a systemic mechanism to spread and colonize different tissues and sites, driving attention to seeds, presenting them as an important niche for survival and dissemination of this pathogen. These results point towards the need of including seed-bearing fruit in studies regarding Xcvi epidemiology. |
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Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapesVitis viniferagrapevine bacterial cankerimmunogold labelingscanning electron microscopyCommercial grapevine fruit (Vitis vinifera) of the Red Globe variety were collected in vineyards from Vale do São Francisco lower basin, an area of occurrence of grapevine bacterial canker. Seeds were extracted, classified as symptomatic or asymptomatic and processed in order to be observed under light (LM) and scanning electron microscopy (SEM) along with silver-enhanced immunogold labeling, to allow bacterial detection using a policlonal antibody against Xanthomonas campestris pv. viticola (Xcvi), etiological agent of the disease. The seed samples showed bacterial aggregates associated to the tegument surface and to the first parenchymal layer beneath the seed tegument. Bacterial identity was confirmed by immunogold labeling. This appears to be the first report of Xcvi associated to asymptomatic seeds and berries, suggesting a systemic mechanism to spread and colonize different tissues and sites, driving attention to seeds, presenting them as an important niche for survival and dissemination of this pathogen. These results point towards the need of including seed-bearing fruit in studies regarding Xcvi epidemiology.Sociedade Brasileira de Fitopatologia2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200004Tropical Plant Pathology v.39 n.2 2014reponame:Tropical plant pathology (Online)instname:Sociedade Brasileira de Fitopatologiainstacron:SBF10.1590/S1982-56762014000200004info:eu-repo/semantics/openAccessTostes,Guilherme de OliveiraAraujo,João Sebastião de PaulaFarias,Antônio Roberto GomesFrade,Daniela Ângela RossinolOlivares,Fábio Lopeseng2014-04-16T00:00:00Zoai:scielo:S1982-56762014000200004Revistahttps://www.scielo.br/j/tpp/ONGhttps://old.scielo.br/oai/scielo-oai.phpsbf-revista@ufla.br1983-20521982-5676opendoar:2014-04-16T00:00Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologiafalse |
dc.title.none.fl_str_mv |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
title |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
spellingShingle |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes Tostes,Guilherme de Oliveira Vitis vinifera grapevine bacterial canker immunogold labeling scanning electron microscopy |
title_short |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
title_full |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
title_fullStr |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
title_full_unstemmed |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
title_sort |
Detection and cellular localization of Xanthomonas campestris pv. viticola in seeds of commercial 'Red Globe' grapes |
author |
Tostes,Guilherme de Oliveira |
author_facet |
Tostes,Guilherme de Oliveira Araujo,João Sebastião de Paula Farias,Antônio Roberto Gomes Frade,Daniela Ângela Rossinol Olivares,Fábio Lopes |
author_role |
author |
author2 |
Araujo,João Sebastião de Paula Farias,Antônio Roberto Gomes Frade,Daniela Ângela Rossinol Olivares,Fábio Lopes |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Tostes,Guilherme de Oliveira Araujo,João Sebastião de Paula Farias,Antônio Roberto Gomes Frade,Daniela Ângela Rossinol Olivares,Fábio Lopes |
dc.subject.por.fl_str_mv |
Vitis vinifera grapevine bacterial canker immunogold labeling scanning electron microscopy |
topic |
Vitis vinifera grapevine bacterial canker immunogold labeling scanning electron microscopy |
description |
Commercial grapevine fruit (Vitis vinifera) of the Red Globe variety were collected in vineyards from Vale do São Francisco lower basin, an area of occurrence of grapevine bacterial canker. Seeds were extracted, classified as symptomatic or asymptomatic and processed in order to be observed under light (LM) and scanning electron microscopy (SEM) along with silver-enhanced immunogold labeling, to allow bacterial detection using a policlonal antibody against Xanthomonas campestris pv. viticola (Xcvi), etiological agent of the disease. The seed samples showed bacterial aggregates associated to the tegument surface and to the first parenchymal layer beneath the seed tegument. Bacterial identity was confirmed by immunogold labeling. This appears to be the first report of Xcvi associated to asymptomatic seeds and berries, suggesting a systemic mechanism to spread and colonize different tissues and sites, driving attention to seeds, presenting them as an important niche for survival and dissemination of this pathogen. These results point towards the need of including seed-bearing fruit in studies regarding Xcvi epidemiology. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1982-56762014000200004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1982-56762014000200004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Fitopatologia |
dc.source.none.fl_str_mv |
Tropical Plant Pathology v.39 n.2 2014 reponame:Tropical plant pathology (Online) instname:Sociedade Brasileira de Fitopatologia instacron:SBF |
instname_str |
Sociedade Brasileira de Fitopatologia |
instacron_str |
SBF |
institution |
SBF |
reponame_str |
Tropical plant pathology (Online) |
collection |
Tropical plant pathology (Online) |
repository.name.fl_str_mv |
Tropical plant pathology (Online) - Sociedade Brasileira de Fitopatologia |
repository.mail.fl_str_mv |
sbf-revista@ufla.br |
_version_ |
1754824586363404288 |