Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000400016 |
Resumo: | Bovine viral diarrhea virus (BVDV) is an etiologic agent that causes important economic losses in the world. It is endemic in cattle herds in most parts of the world. The purpose of this study was to evaluate the in vitro cytotoxic effect and antiviral properties of several marine natural products obtained from seaweeds: the indole alkaloid caulerpin (CAV, 1) and three diterpenes: 6-hydroxydichotoma-3,14-diene-1,17-dial (DA, 2), 10,18-diacetoxy-8-hydroxy-2,6-dolabelladiene (DB1, 3) and 8,10,18-trihydroxy-2,6-dolabelladiene (DB3, 4). The screening to evaluate the cytotoxicity of compounds did not show toxic effects to MDBK cells. The antiviral activity of the compounds was measured by the inhibition of the cytopathic effect on infected cells by plaque assay (PA) and EC50 values were calculated for CAV (EC=2,0± 5.8), DA (EC 2,8± 7.7), DB1 (EC 2,0±9.7), and DB3 (EC 2,3±7.4). Acyclovir (EC50 322± 5.9) was used in all experiments as the control standard. Although the results of the antiviral activity suggest that all compounds are promising as antiviral agents against BVDV, the Selectivity Index suggests that DB1 is the safest of the compounds tested. |
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Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virusBVDVantiviralcaulerpinditerpenesmarine algaeCaulerpaDictyotaBovine viral diarrhea virus (BVDV) is an etiologic agent that causes important economic losses in the world. It is endemic in cattle herds in most parts of the world. The purpose of this study was to evaluate the in vitro cytotoxic effect and antiviral properties of several marine natural products obtained from seaweeds: the indole alkaloid caulerpin (CAV, 1) and three diterpenes: 6-hydroxydichotoma-3,14-diene-1,17-dial (DA, 2), 10,18-diacetoxy-8-hydroxy-2,6-dolabelladiene (DB1, 3) and 8,10,18-trihydroxy-2,6-dolabelladiene (DB3, 4). The screening to evaluate the cytotoxicity of compounds did not show toxic effects to MDBK cells. The antiviral activity of the compounds was measured by the inhibition of the cytopathic effect on infected cells by plaque assay (PA) and EC50 values were calculated for CAV (EC=2,0± 5.8), DA (EC 2,8± 7.7), DB1 (EC 2,0±9.7), and DB3 (EC 2,3±7.4). Acyclovir (EC50 322± 5.9) was used in all experiments as the control standard. Although the results of the antiviral activity suggest that all compounds are promising as antiviral agents against BVDV, the Selectivity Index suggests that DB1 is the safest of the compounds tested.Sociedade Brasileira de Farmacognosia2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000400016Revista Brasileira de Farmacognosia v.22 n.4 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2012005000060info:eu-repo/semantics/openAccessPinto,Ana Maria VianaLeite,José Paulo G.Ferreira,Wilton J.Cavalcanti,Diana N.Villaça,Roberto C.Giongo,VivecaTeixeira,Valéria L.Paixão,Izabel Christina Nunes de Palmereng2012-06-27T00:00:00Zoai:scielo:S0102-695X2012000400016Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-06-27T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
title |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
spellingShingle |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus Pinto,Ana Maria Viana BVDV antiviral caulerpin diterpenes marine algae Caulerpa Dictyota |
title_short |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
title_full |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
title_fullStr |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
title_full_unstemmed |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
title_sort |
Marine natural seaweed products as potential antiviral drugs against Bovine viral diarrhea virus |
author |
Pinto,Ana Maria Viana |
author_facet |
Pinto,Ana Maria Viana Leite,José Paulo G. Ferreira,Wilton J. Cavalcanti,Diana N. Villaça,Roberto C. Giongo,Viveca Teixeira,Valéria L. Paixão,Izabel Christina Nunes de Palmer |
author_role |
author |
author2 |
Leite,José Paulo G. Ferreira,Wilton J. Cavalcanti,Diana N. Villaça,Roberto C. Giongo,Viveca Teixeira,Valéria L. Paixão,Izabel Christina Nunes de Palmer |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pinto,Ana Maria Viana Leite,José Paulo G. Ferreira,Wilton J. Cavalcanti,Diana N. Villaça,Roberto C. Giongo,Viveca Teixeira,Valéria L. Paixão,Izabel Christina Nunes de Palmer |
dc.subject.por.fl_str_mv |
BVDV antiviral caulerpin diterpenes marine algae Caulerpa Dictyota |
topic |
BVDV antiviral caulerpin diterpenes marine algae Caulerpa Dictyota |
description |
Bovine viral diarrhea virus (BVDV) is an etiologic agent that causes important economic losses in the world. It is endemic in cattle herds in most parts of the world. The purpose of this study was to evaluate the in vitro cytotoxic effect and antiviral properties of several marine natural products obtained from seaweeds: the indole alkaloid caulerpin (CAV, 1) and three diterpenes: 6-hydroxydichotoma-3,14-diene-1,17-dial (DA, 2), 10,18-diacetoxy-8-hydroxy-2,6-dolabelladiene (DB1, 3) and 8,10,18-trihydroxy-2,6-dolabelladiene (DB3, 4). The screening to evaluate the cytotoxicity of compounds did not show toxic effects to MDBK cells. The antiviral activity of the compounds was measured by the inhibition of the cytopathic effect on infected cells by plaque assay (PA) and EC50 values were calculated for CAV (EC=2,0± 5.8), DA (EC 2,8± 7.7), DB1 (EC 2,0±9.7), and DB3 (EC 2,3±7.4). Acyclovir (EC50 322± 5.9) was used in all experiments as the control standard. Although the results of the antiviral activity suggest that all compounds are promising as antiviral agents against BVDV, the Selectivity Index suggests that DB1 is the safest of the compounds tested. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000400016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000400016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2012005000060 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.22 n.4 2012 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122467507240960 |