A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction

Detalhes bibliográficos
Autor(a) principal: Santelli,Roberto V.
Data de Publicação: 2000
Outros Autores: Navarro-Cattapan,Luci Deise
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011
Resumo: A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
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spelling A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extractionA modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.Sociedade Brasileira de Genética2000-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011Genetics and Molecular Biology v.23 n.1 2000reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572000000100011info:eu-repo/semantics/openAccessSantelli,Roberto V.Navarro-Cattapan,Luci Deiseeng2000-08-25T00:00:00Zoai:scielo:S1415-47572000000100011Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2000-08-25T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
title A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
spellingShingle A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
Santelli,Roberto V.
title_short A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
title_full A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
title_fullStr A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
title_full_unstemmed A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
title_sort A simplified CsCL protocol for lambda DNA purification: no enzymatic treatment/one phenol extraction
author Santelli,Roberto V.
author_facet Santelli,Roberto V.
Navarro-Cattapan,Luci Deise
author_role author
author2 Navarro-Cattapan,Luci Deise
author2_role author
dc.contributor.author.fl_str_mv Santelli,Roberto V.
Navarro-Cattapan,Luci Deise
description A modification of the CsCl gradient centrifugation method for DNA phage purification is presented. It avoids the enzymatic steps as well the need for a preliminary phage titration, a tedious process proposed in the majority of the protocols in use. The quality of the DNA obtained makes it amenable for additional manipulations like digestions, ligations, labelling, subcloning, etc.
publishDate 2000
dc.date.none.fl_str_mv 2000-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572000000100011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.23 n.1 2000
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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