Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100012 |
Resumo: | The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - S J) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - S J = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - S J = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - S J = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed. |
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Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivarsbarleyamylasesisoenzymesstructuralregulatory genetic differencesThe amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - S J) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - S J = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - S J = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - S J = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed.Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100012Genetics and Molecular Biology v.26 n.1 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000100012info:eu-repo/semantics/openAccessGeorg-Kraemer,J.E.Cavalli,S.S.eng2003-09-05T00:00:00Zoai:scielo:S1415-47572003000100012Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-09-05T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
spellingShingle |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars Georg-Kraemer,J.E. barley amylases isoenzymes structural regulatory genetic differences |
title_short |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_full |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_fullStr |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_full_unstemmed |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_sort |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
author |
Georg-Kraemer,J.E. |
author_facet |
Georg-Kraemer,J.E. Cavalli,S.S. |
author_role |
author |
author2 |
Cavalli,S.S. |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Georg-Kraemer,J.E. Cavalli,S.S. |
dc.subject.por.fl_str_mv |
barley amylases isoenzymes structural regulatory genetic differences |
topic |
barley amylases isoenzymes structural regulatory genetic differences |
description |
The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - S J) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - S J = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - S J = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - S J = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572003000100012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.26 n.1 2003 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122378593239040 |