Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022 |
Resumo: | The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees. |
id |
SBG-1_5ea4823db8b413965a399b97f677da12 |
---|---|
oai_identifier_str |
oai:scielo:S1415-47572013000200022 |
network_acronym_str |
SBG-1 |
network_name_str |
Genetics and Molecular Biology |
repository_id_str |
|
spelling |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptomefat bodygene expressionMeliponastingless beestranscriptomeThe insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees.Sociedade Brasileira de Genética2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022Genetics and Molecular Biology v.36 n.2 2013reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572013000200022info:eu-repo/semantics/openAccessSousa,Cristina Soares deSerrão,José EduardoBonetti,Ana MariaAmaral,Isabel Marques RodriguesKerr,Warwick EstevamMaranhão,Andréa QueirozUeira-Vieira,Carloseng2013-06-07T00:00:00Zoai:scielo:S1415-47572013000200022Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2013-06-07T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
title |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
spellingShingle |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome Sousa,Cristina Soares de fat body gene expression Melipona stingless bees transcriptome |
title_short |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
title_full |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
title_fullStr |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
title_full_unstemmed |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
title_sort |
Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome |
author |
Sousa,Cristina Soares de |
author_facet |
Sousa,Cristina Soares de Serrão,José Eduardo Bonetti,Ana Maria Amaral,Isabel Marques Rodrigues Kerr,Warwick Estevam Maranhão,Andréa Queiroz Ueira-Vieira,Carlos |
author_role |
author |
author2 |
Serrão,José Eduardo Bonetti,Ana Maria Amaral,Isabel Marques Rodrigues Kerr,Warwick Estevam Maranhão,Andréa Queiroz Ueira-Vieira,Carlos |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sousa,Cristina Soares de Serrão,José Eduardo Bonetti,Ana Maria Amaral,Isabel Marques Rodrigues Kerr,Warwick Estevam Maranhão,Andréa Queiroz Ueira-Vieira,Carlos |
dc.subject.por.fl_str_mv |
fat body gene expression Melipona stingless bees transcriptome |
topic |
fat body gene expression Melipona stingless bees transcriptome |
description |
The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in stingless bees. In this study, we analyzed the transcriptome of the fat body from the stingless bee Melipona scutellaris. In silico analysis of a set of cDNA library sequences yielded 1728 expressed sequence tags (ESTs) and 997 high-quality sequences that were assembled into 29 contigs and 117 singlets. The BLAST X tool showed that 86% of the ESTs shared similarity with Apis mellifera (honeybee) genes. The M. scutellaris fat body ESTs encoded proteins with roles in numerous physiological processes, including anti-oxidation, phosphorylation, metabolism, detoxification, transmembrane transport, intracellular transport, cell proliferation, protein hydrolysis and protein synthesis. This is the first report to describe a transcriptomic analysis of specific organs of M. scutellaris. Our findings provide new insights into the physiological role of the fat body in stingless bees. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572013000200022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.36 n.2 2013 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
_version_ |
1752122385428905984 |