Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers

Detalhes bibliográficos
Autor(a) principal: Silveira,Sheila Recepute
Data de Publicação: 2003
Outros Autores: Ruas,Paulo Maurício, Ruas,Claudete de Fátima, Sera,Tumoru, Carvalho,Valdemar de Paula, Coelho,Alexandre Siqueira Guedes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018
Resumo: The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding.
id SBG-1_8ced7858867c28e9296f6e5619e94664
oai_identifier_str oai:scielo:S1415-47572003000300018
network_acronym_str SBG-1
network_name_str Genetics and Molecular Biology
repository_id_str
spelling Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markersCoffee breedinggenetic variabilitymolecular varianceRAPD markersThe RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding.Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018Genetics and Molecular Biology v.26 n.3 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000300018info:eu-repo/semantics/openAccessSilveira,Sheila ReceputeRuas,Paulo MaurícioRuas,Claudete de FátimaSera,TumoruCarvalho,Valdemar de PaulaCoelho,Alexandre Siqueira Guedeseng2003-09-29T00:00:00Zoai:scielo:S1415-47572003000300018Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-09-29T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
title Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
spellingShingle Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
Silveira,Sheila Recepute
Coffee breeding
genetic variability
molecular variance
RAPD markers
title_short Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
title_full Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
title_fullStr Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
title_full_unstemmed Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
title_sort Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers
author Silveira,Sheila Recepute
author_facet Silveira,Sheila Recepute
Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Sera,Tumoru
Carvalho,Valdemar de Paula
Coelho,Alexandre Siqueira Guedes
author_role author
author2 Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Sera,Tumoru
Carvalho,Valdemar de Paula
Coelho,Alexandre Siqueira Guedes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Silveira,Sheila Recepute
Ruas,Paulo Maurício
Ruas,Claudete de Fátima
Sera,Tumoru
Carvalho,Valdemar de Paula
Coelho,Alexandre Siqueira Guedes
dc.subject.por.fl_str_mv Coffee breeding
genetic variability
molecular variance
RAPD markers
topic Coffee breeding
genetic variability
molecular variance
RAPD markers
description The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572003000300018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.26 n.3 2003
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
_version_ 1752122378664542208

Registros relacionados