Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide

Detalhes bibliográficos
Autor(a) principal: Becker,Tânia C.A.
Data de Publicação: 2003
Outros Autores: Chiuchetta,Simone J.R., Baptista,Francielle, Castro-Prado,Marialba A.A. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026
Resumo: Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.
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spelling Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromideantineoplastic drugAspergillus nidulansethidium bromideintercalating agentmitotic crossing overEthidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.Sociedade Brasileira de Genética2003-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026Genetics and Molecular Biology v.26 n.3 2003reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572003000300026info:eu-repo/semantics/openAccessBecker,Tânia C.A.Chiuchetta,Simone J.R.Baptista,FrancielleCastro-Prado,Marialba A.A. deeng2003-09-29T00:00:00Zoai:scielo:S1415-47572003000300026Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2003-09-29T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
title Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
spellingShingle Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
Becker,Tânia C.A.
antineoplastic drug
Aspergillus nidulans
ethidium bromide
intercalating agent
mitotic crossing over
title_short Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
title_full Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
title_fullStr Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
title_full_unstemmed Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
title_sort Increase in mitotic recombination in diploid cells of Aspergillus nidulans in response to ethidium bromide
author Becker,Tânia C.A.
author_facet Becker,Tânia C.A.
Chiuchetta,Simone J.R.
Baptista,Francielle
Castro-Prado,Marialba A.A. de
author_role author
author2 Chiuchetta,Simone J.R.
Baptista,Francielle
Castro-Prado,Marialba A.A. de
author2_role author
author
author
dc.contributor.author.fl_str_mv Becker,Tânia C.A.
Chiuchetta,Simone J.R.
Baptista,Francielle
Castro-Prado,Marialba A.A. de
dc.subject.por.fl_str_mv antineoplastic drug
Aspergillus nidulans
ethidium bromide
intercalating agent
mitotic crossing over
topic antineoplastic drug
Aspergillus nidulans
ethidium bromide
intercalating agent
mitotic crossing over
description Ethidium bromide (EB) is an intercalating inhibitor of topoisomerase II and its activities are related to chemotherapeutic drugs used in anti-cancer treatments. EB promotes several genotoxic effects in exposed cells by stabilising the DNA-enzyme complex. The recombinagenic potential of EB was evaluated in our in vivo study by the loss of heterozygosity of nutritional markers in diploid Aspergillus nidulans cells through Homozygotization Index (HI). A DNA repair mutation, uvsZ and a chromosome duplication DP (II-I) were introduced in the genome of tested cells to obtain a sensitive system for the recombinagenesis detection. EB-treated diploid cells had HI values significantly greater than the control at both concentrations (4.0 x 10-3 and 5.0 x 10-3 mM). Results indicate that the intercalating agent is potentially capable of inducing mitotic crossing-over in diploid A. nidulans cells.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572003000300026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.26 n.3 2003
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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