Eucalyptus urograndis stem proteome is responsive to short-term cold stress

Detalhes bibliográficos
Autor(a) principal: Leonardi,Gabriela de Almeida
Data de Publicação: 2015
Outros Autores: Carlos,Natália Aparecida, Mazzafera,Paulo, Balbuena,Tiago Santana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191
Resumo: Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.
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spelling Eucalyptus urograndis stem proteome is responsive to short-term cold stressabiotic stressmass spectrometryomicsproteomicsEucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.Sociedade Brasileira de Genética2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191Genetics and Molecular Biology v.38 n.2 2015reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-475738220140235info:eu-repo/semantics/openAccessLeonardi,Gabriela de AlmeidaCarlos,Natália AparecidaMazzafera,PauloBalbuena,Tiago Santanaeng2015-07-31T00:00:00Zoai:scielo:S1415-47572015000200191Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2015-07-31T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title Eucalyptus urograndis stem proteome is responsive to short-term cold stress
spellingShingle Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Leonardi,Gabriela de Almeida
abiotic stress
mass spectrometry
omics
proteomics
title_short Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_full Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_fullStr Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_full_unstemmed Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_sort Eucalyptus urograndis stem proteome is responsive to short-term cold stress
author Leonardi,Gabriela de Almeida
author_facet Leonardi,Gabriela de Almeida
Carlos,Natália Aparecida
Mazzafera,Paulo
Balbuena,Tiago Santana
author_role author
author2 Carlos,Natália Aparecida
Mazzafera,Paulo
Balbuena,Tiago Santana
author2_role author
author
author
dc.contributor.author.fl_str_mv Leonardi,Gabriela de Almeida
Carlos,Natália Aparecida
Mazzafera,Paulo
Balbuena,Tiago Santana
dc.subject.por.fl_str_mv abiotic stress
mass spectrometry
omics
proteomics
topic abiotic stress
mass spectrometry
omics
proteomics
description Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-475738220140235
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.38 n.2 2015
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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