Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/129168 |
Resumo: | Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress. |
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Repositório Institucional da UNESP |
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Eucalyptus urograndis stem proteome is responsive to short-term cold stressAbiotic stressMass spectrometryOmicsProteomicsEucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, BrazilDepartamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Jaboticabal, SP, BrazilFAPESP: 2011/11650-0FAPESP: 2011/51949-5FAPESP: 2013/06370-4FAPESP: 2013/06352-6FAPESP: 2011/23582-0Soc Brasil GeneticaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Leonardi, Gabriela de Almeida [UNESP]Carlos, Natália Aparecida [UNESP]Mazzafera, PauloBalbuena, Tiago Santana [UNESP]2015-10-21T20:30:05Z2015-10-21T20:30:05Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article191-198application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=enGenetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015.1415-4757http://hdl.handle.net/11449/12916810.1590/S1415-475738220140235S1415-47572015000200191S1415-47572015000200191.pdf2767178161293127SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-06-07T15:31:46Zoai:repositorio.unesp.br:11449/129168Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:00.681901Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
title |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
spellingShingle |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress Leonardi, Gabriela de Almeida [UNESP] Abiotic stress Mass spectrometry Omics Proteomics |
title_short |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
title_full |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
title_fullStr |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
title_full_unstemmed |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
title_sort |
Eucalyptus urograndis stem proteome is responsive to short-term cold stress |
author |
Leonardi, Gabriela de Almeida [UNESP] |
author_facet |
Leonardi, Gabriela de Almeida [UNESP] Carlos, Natália Aparecida [UNESP] Mazzafera, Paulo Balbuena, Tiago Santana [UNESP] |
author_role |
author |
author2 |
Carlos, Natália Aparecida [UNESP] Mazzafera, Paulo Balbuena, Tiago Santana [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Leonardi, Gabriela de Almeida [UNESP] Carlos, Natália Aparecida [UNESP] Mazzafera, Paulo Balbuena, Tiago Santana [UNESP] |
dc.subject.por.fl_str_mv |
Abiotic stress Mass spectrometry Omics Proteomics |
topic |
Abiotic stress Mass spectrometry Omics Proteomics |
description |
Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-21T20:30:05Z 2015-10-21T20:30:05Z 2015-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015. 1415-4757 http://hdl.handle.net/11449/129168 10.1590/S1415-475738220140235 S1415-47572015000200191 S1415-47572015000200191.pdf 2767178161293127 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/129168 |
identifier_str_mv |
Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015. 1415-4757 10.1590/S1415-475738220140235 S1415-47572015000200191 S1415-47572015000200191.pdf 2767178161293127 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics And Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
191-198 application/pdf |
dc.publisher.none.fl_str_mv |
Soc Brasil Genetica |
publisher.none.fl_str_mv |
Soc Brasil Genetica |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128791532273664 |