Eucalyptus urograndis stem proteome is responsive to short-term cold stress

Detalhes bibliográficos
Autor(a) principal: Leonardi, Gabriela de Almeida [UNESP]
Data de Publicação: 2015
Outros Autores: Carlos, Natália Aparecida [UNESP], Mazzafera, Paulo, Balbuena, Tiago Santana [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129168
Resumo: Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.
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spelling Eucalyptus urograndis stem proteome is responsive to short-term cold stressAbiotic stressMass spectrometryOmicsProteomicsEucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, BrazilDepartamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista “Júlio de Mesquita Filho”, Jaboticabal, SP, BrazilFAPESP: 2011/11650-0FAPESP: 2011/51949-5FAPESP: 2013/06370-4FAPESP: 2013/06352-6FAPESP: 2011/23582-0Soc Brasil GeneticaUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Leonardi, Gabriela de Almeida [UNESP]Carlos, Natália Aparecida [UNESP]Mazzafera, PauloBalbuena, Tiago Santana [UNESP]2015-10-21T20:30:05Z2015-10-21T20:30:05Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article191-198application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=enGenetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015.1415-4757http://hdl.handle.net/11449/12916810.1590/S1415-475738220140235S1415-47572015000200191S1415-47572015000200191.pdf2767178161293127SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics And Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2024-06-07T15:31:46Zoai:repositorio.unesp.br:11449/129168Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:00.681901Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title Eucalyptus urograndis stem proteome is responsive to short-term cold stress
spellingShingle Eucalyptus urograndis stem proteome is responsive to short-term cold stress
Leonardi, Gabriela de Almeida [UNESP]
Abiotic stress
Mass spectrometry
Omics
Proteomics
title_short Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_full Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_fullStr Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_full_unstemmed Eucalyptus urograndis stem proteome is responsive to short-term cold stress
title_sort Eucalyptus urograndis stem proteome is responsive to short-term cold stress
author Leonardi, Gabriela de Almeida [UNESP]
author_facet Leonardi, Gabriela de Almeida [UNESP]
Carlos, Natália Aparecida [UNESP]
Mazzafera, Paulo
Balbuena, Tiago Santana [UNESP]
author_role author
author2 Carlos, Natália Aparecida [UNESP]
Mazzafera, Paulo
Balbuena, Tiago Santana [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Estadual de Campinas (UNICAMP)
dc.contributor.author.fl_str_mv Leonardi, Gabriela de Almeida [UNESP]
Carlos, Natália Aparecida [UNESP]
Mazzafera, Paulo
Balbuena, Tiago Santana [UNESP]
dc.subject.por.fl_str_mv Abiotic stress
Mass spectrometry
Omics
Proteomics
topic Abiotic stress
Mass spectrometry
Omics
Proteomics
description Eucalyptus urograndis is a hybrid eucalyptus of major economic importance to the Brazilian pulp and paper industry. Although widely used in forest nurseries around the country, little is known about the biochemical changes imposed by environmental stress in this species. In this study, we evaluated the changes in the stem proteome after short-term stimulation by exposure to low temperature. Using two-dimensional gel electrophoresis coupled to high-resolution mass spectrometry-based protein identification, 12 proteins were found to be differentially regulated and successfully identified after stringent database searches against a protein database from a closely related species (Eucalyptus grandis). The identification of these proteins indicated that the E. urograndis stem proteome responded quickly to low temperature, mostly by down-regulating specific proteins involved in energy metabolism, protein synthesis and signaling. The results of this study represent the first step in understanding the molecular and biochemical responses of E. urograndis to thermal stress.
publishDate 2015
dc.date.none.fl_str_mv 2015-10-21T20:30:05Z
2015-10-21T20:30:05Z
2015-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en
Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015.
1415-4757
http://hdl.handle.net/11449/129168
10.1590/S1415-475738220140235
S1415-47572015000200191
S1415-47572015000200191.pdf
2767178161293127
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200191&lng=en&nrm=iso&tlng=en
http://hdl.handle.net/11449/129168
identifier_str_mv Genetics And Molecular Biology. Ribeirao Pret: Soc Brasil Genetica, v. 38, n. 2, p. 191-198, 2015.
1415-4757
10.1590/S1415-475738220140235
S1415-47572015000200191
S1415-47572015000200191.pdf
2767178161293127
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics And Molecular Biology
1.493
0,638
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 191-198
application/pdf
dc.publisher.none.fl_str_mv Soc Brasil Genetica
publisher.none.fl_str_mv Soc Brasil Genetica
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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