A proteomic study of mesenchymal stem cells from equine umbilical cord

Detalhes bibliográficos
Autor(a) principal: Maia, Leandro [UNESP]
Data de Publicação: 2017
Outros Autores: de Moraes, Carolina Nogueira [UNESP], Dias, Marianne Camargos [UNESP], Martinez, Julia Bauzá, Caballol, Antonia Odena, Testoni, Giorgia, de Queiroz, Carla Martins [UNESP], Peña, Ramón Díaz, Landim-Alvarenga, Fernanda C. [UNESP], de Oliveira, Eliandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2017.05.015
http://hdl.handle.net/11449/174711
Resumo: To the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes.
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spelling A proteomic study of mesenchymal stem cells from equine umbilical cordHorseMass spectrometryMSCsProteinProteomicsTo the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University UNESPProteomics Platform Parc Cientific de Barcelona (PCB)Institute for Research in Biomedicine (IRB)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University UNESPFAPESP: 12/23888-4FAPESP: 15/17619-9Universidade Estadual Paulista (Unesp)Parc Cientific de Barcelona (PCB)Institute for Research in Biomedicine (IRB)Maia, Leandro [UNESP]de Moraes, Carolina Nogueira [UNESP]Dias, Marianne Camargos [UNESP]Martinez, Julia BauzáCaballol, Antonia OdenaTestoni, Giorgiade Queiroz, Carla Martins [UNESP]Peña, Ramón DíazLandim-Alvarenga, Fernanda C. [UNESP]de Oliveira, Eliandre2018-12-11T17:12:32Z2018-12-11T17:12:32Z2017-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8-15application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2017.05.015Theriogenology, v. 100, p. 8-15.0093-691Xhttp://hdl.handle.net/11449/17471110.1016/j.theriogenology.2017.05.0152-s2.0-850202434842-s2.0-85020243484.pdf84564903008148330000-0002-2420-2550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:05:36Zoai:repositorio.unesp.br:11449/174711Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A proteomic study of mesenchymal stem cells from equine umbilical cord
title A proteomic study of mesenchymal stem cells from equine umbilical cord
spellingShingle A proteomic study of mesenchymal stem cells from equine umbilical cord
Maia, Leandro [UNESP]
Horse
Mass spectrometry
MSCs
Protein
Proteomics
title_short A proteomic study of mesenchymal stem cells from equine umbilical cord
title_full A proteomic study of mesenchymal stem cells from equine umbilical cord
title_fullStr A proteomic study of mesenchymal stem cells from equine umbilical cord
title_full_unstemmed A proteomic study of mesenchymal stem cells from equine umbilical cord
title_sort A proteomic study of mesenchymal stem cells from equine umbilical cord
author Maia, Leandro [UNESP]
author_facet Maia, Leandro [UNESP]
de Moraes, Carolina Nogueira [UNESP]
Dias, Marianne Camargos [UNESP]
Martinez, Julia Bauzá
Caballol, Antonia Odena
Testoni, Giorgia
de Queiroz, Carla Martins [UNESP]
Peña, Ramón Díaz
Landim-Alvarenga, Fernanda C. [UNESP]
de Oliveira, Eliandre
author_role author
author2 de Moraes, Carolina Nogueira [UNESP]
Dias, Marianne Camargos [UNESP]
Martinez, Julia Bauzá
Caballol, Antonia Odena
Testoni, Giorgia
de Queiroz, Carla Martins [UNESP]
Peña, Ramón Díaz
Landim-Alvarenga, Fernanda C. [UNESP]
de Oliveira, Eliandre
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Parc Cientific de Barcelona (PCB)
Institute for Research in Biomedicine (IRB)
dc.contributor.author.fl_str_mv Maia, Leandro [UNESP]
de Moraes, Carolina Nogueira [UNESP]
Dias, Marianne Camargos [UNESP]
Martinez, Julia Bauzá
Caballol, Antonia Odena
Testoni, Giorgia
de Queiroz, Carla Martins [UNESP]
Peña, Ramón Díaz
Landim-Alvarenga, Fernanda C. [UNESP]
de Oliveira, Eliandre
dc.subject.por.fl_str_mv Horse
Mass spectrometry
MSCs
Protein
Proteomics
topic Horse
Mass spectrometry
MSCs
Protein
Proteomics
description To the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes.
publishDate 2017
dc.date.none.fl_str_mv 2017-09-15
2018-12-11T17:12:32Z
2018-12-11T17:12:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2017.05.015
Theriogenology, v. 100, p. 8-15.
0093-691X
http://hdl.handle.net/11449/174711
10.1016/j.theriogenology.2017.05.015
2-s2.0-85020243484
2-s2.0-85020243484.pdf
8456490300814833
0000-0002-2420-2550
url http://dx.doi.org/10.1016/j.theriogenology.2017.05.015
http://hdl.handle.net/11449/174711
identifier_str_mv Theriogenology, v. 100, p. 8-15.
0093-691X
10.1016/j.theriogenology.2017.05.015
2-s2.0-85020243484
2-s2.0-85020243484.pdf
8456490300814833
0000-0002-2420-2550
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8-15
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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