A proteomic study of mesenchymal stem cells from equine umbilical cord
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2017.05.015 http://hdl.handle.net/11449/174711 |
Resumo: | To the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes. |
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A proteomic study of mesenchymal stem cells from equine umbilical cordHorseMass spectrometryMSCsProteinProteomicsTo the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University UNESPProteomics Platform Parc Cientific de Barcelona (PCB)Institute for Research in Biomedicine (IRB)Department of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Science São Paulo State University UNESPFAPESP: 12/23888-4FAPESP: 15/17619-9Universidade Estadual Paulista (Unesp)Parc Cientific de Barcelona (PCB)Institute for Research in Biomedicine (IRB)Maia, Leandro [UNESP]de Moraes, Carolina Nogueira [UNESP]Dias, Marianne Camargos [UNESP]Martinez, Julia BauzáCaballol, Antonia OdenaTestoni, Giorgiade Queiroz, Carla Martins [UNESP]Peña, Ramón DíazLandim-Alvarenga, Fernanda C. [UNESP]de Oliveira, Eliandre2018-12-11T17:12:32Z2018-12-11T17:12:32Z2017-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8-15application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2017.05.015Theriogenology, v. 100, p. 8-15.0093-691Xhttp://hdl.handle.net/11449/17471110.1016/j.theriogenology.2017.05.0152-s2.0-850202434842-s2.0-85020243484.pdf84564903008148330000-0002-2420-2550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:05:36Zoai:repositorio.unesp.br:11449/174711Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
title |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
spellingShingle |
A proteomic study of mesenchymal stem cells from equine umbilical cord Maia, Leandro [UNESP] Horse Mass spectrometry MSCs Protein Proteomics |
title_short |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
title_full |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
title_fullStr |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
title_full_unstemmed |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
title_sort |
A proteomic study of mesenchymal stem cells from equine umbilical cord |
author |
Maia, Leandro [UNESP] |
author_facet |
Maia, Leandro [UNESP] de Moraes, Carolina Nogueira [UNESP] Dias, Marianne Camargos [UNESP] Martinez, Julia Bauzá Caballol, Antonia Odena Testoni, Giorgia de Queiroz, Carla Martins [UNESP] Peña, Ramón Díaz Landim-Alvarenga, Fernanda C. [UNESP] de Oliveira, Eliandre |
author_role |
author |
author2 |
de Moraes, Carolina Nogueira [UNESP] Dias, Marianne Camargos [UNESP] Martinez, Julia Bauzá Caballol, Antonia Odena Testoni, Giorgia de Queiroz, Carla Martins [UNESP] Peña, Ramón Díaz Landim-Alvarenga, Fernanda C. [UNESP] de Oliveira, Eliandre |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Parc Cientific de Barcelona (PCB) Institute for Research in Biomedicine (IRB) |
dc.contributor.author.fl_str_mv |
Maia, Leandro [UNESP] de Moraes, Carolina Nogueira [UNESP] Dias, Marianne Camargos [UNESP] Martinez, Julia Bauzá Caballol, Antonia Odena Testoni, Giorgia de Queiroz, Carla Martins [UNESP] Peña, Ramón Díaz Landim-Alvarenga, Fernanda C. [UNESP] de Oliveira, Eliandre |
dc.subject.por.fl_str_mv |
Horse Mass spectrometry MSCs Protein Proteomics |
topic |
Horse Mass spectrometry MSCs Protein Proteomics |
description |
To the best of our knowledge, this is the first study describing the proteome of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs) in a global and functional manner. The aim of this work was to analyze the proteome of previously characterized UCIM-MSCs to determine protein abundance and classify the identified proteins according to Gene Ontology (GO) terms. Protein classification analysis according to biological process, molecular function and cellular component was performed using the PANTHER (Protein ANalysis THrough Evolutionary Relationships) Classification System, which revealed enrichment for 42 biological processes, 23 molecular functions and 18 cellular components. Protein abundance was estimated according to the emPAI method (Exponential Modified Protein Abundance Index). The two most abundant proteins in the proteome of UCIM-MSCs were the cytoskeletal proteins actin and vimentin, which have important roles in cell stability and motility. Additionally, we identified 14 cell surface antigens. Three of them, CD44, CD90 and CD105, had been previously validated by flow cytometry. In the present study, we also identified important information about the biological properties of UCIM-MSCs such as differentiation potential, low immunogenicity (low MHC-II expression) and chromosomal stability, which reinforces their use for cell therapy. Together with the proteomic findings, this information allowed us to infer the functional relevance of several activities related to primary metabolic processes, protein synthesis, production of vesicle coats, vesicle-mediated transport and antioxidant activity. In addition, the identification of different cell surface markers may help establish an immunophenotypic panel suitable for the characterization of MSCs from equine fetal membranes. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-09-15 2018-12-11T17:12:32Z 2018-12-11T17:12:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2017.05.015 Theriogenology, v. 100, p. 8-15. 0093-691X http://hdl.handle.net/11449/174711 10.1016/j.theriogenology.2017.05.015 2-s2.0-85020243484 2-s2.0-85020243484.pdf 8456490300814833 0000-0002-2420-2550 |
url |
http://dx.doi.org/10.1016/j.theriogenology.2017.05.015 http://hdl.handle.net/11449/174711 |
identifier_str_mv |
Theriogenology, v. 100, p. 8-15. 0093-691X 10.1016/j.theriogenology.2017.05.015 2-s2.0-85020243484 2-s2.0-85020243484.pdf 8456490300814833 0000-0002-2420-2550 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8-15 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546596573380608 |