Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Genetics and Molecular Biology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019 |
Resumo: | We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype. |
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Genetics and Molecular Biology |
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Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNACestrumchromosome bandingCSRskaryotype evolutionrDNA probesWe studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype.Sociedade Brasileira de Genética2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019Genetics and Molecular Biology v.29 n.1 2006reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572006000100019info:eu-repo/semantics/openAccessFregonezi,Jéferson NunesFernandes,ThiagoTorezan,José Marcelo DominguesVieira,Ana Odete S.Vanzela,André Luís Laforgaeng2006-03-10T00:00:00Zoai:scielo:S1415-47572006000100019Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2006-03-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false |
dc.title.none.fl_str_mv |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
title |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
spellingShingle |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA Fregonezi,Jéferson Nunes Cestrum chromosome banding CSRs karyotype evolution rDNA probes |
title_short |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
title_full |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
title_fullStr |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
title_full_unstemmed |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
title_sort |
Karyotype differentiation of four Cestrum species (Solanaceae) based on the physical mapping of repetitive DNA |
author |
Fregonezi,Jéferson Nunes |
author_facet |
Fregonezi,Jéferson Nunes Fernandes,Thiago Torezan,José Marcelo Domingues Vieira,Ana Odete S. Vanzela,André Luís Laforga |
author_role |
author |
author2 |
Fernandes,Thiago Torezan,José Marcelo Domingues Vieira,Ana Odete S. Vanzela,André Luís Laforga |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Fregonezi,Jéferson Nunes Fernandes,Thiago Torezan,José Marcelo Domingues Vieira,Ana Odete S. Vanzela,André Luís Laforga |
dc.subject.por.fl_str_mv |
Cestrum chromosome banding CSRs karyotype evolution rDNA probes |
topic |
Cestrum chromosome banding CSRs karyotype evolution rDNA probes |
description |
We studied the karyotypes of four Brazilian Cestrum species (C. amictum, C. intermedium, C. sendtnerianum and C. strigilatum) using conventional Feulgen staining, C-Giemsa and C-CMA3/DAPI banding, induction of cold-sensitive regions (CSRs) and fluorescent in situ hybridization (FISH) with rDNA probes. We found that the karyotypes of all four species was 2n = 2x = 16, with, except for the eighth acrocentric pair, a predominance of meta- and submetacentric chromosomes and various heterochromatin classes. Heterochromatic types previously unreported in Cestrum as neutral C-CMA3(0)/DAPI0 bands, CMA3+ bands not associated with NORs, and C-Giemsa/CSR/DAPI- bands were found. The heterochromatic blocks varied in size, number, position and composition. The 45S rDNA probe preferentially located in the terminal and subterminal regions of some chromosomes, while 5S rDNA appeared close to the centromere of the long arm of pair 8. These results suggest that karyotype differentiation can occur mainly due to changes in repetitive DNA, with little modification in the general composition of the conventionally stained karyotype. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1415-47572006000100019 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
dc.source.none.fl_str_mv |
Genetics and Molecular Biology v.29 n.1 2006 reponame:Genetics and Molecular Biology instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
instname_str |
Sociedade Brasileira de Genética (SBG) |
instacron_str |
SBG |
institution |
SBG |
reponame_str |
Genetics and Molecular Biology |
collection |
Genetics and Molecular Biology |
repository.name.fl_str_mv |
Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG) |
repository.mail.fl_str_mv |
||editor@gmb.org.br |
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1752122379867258880 |