Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification

Detalhes bibliográficos
Autor(a) principal: Souza-Shibatta,Lenice
Data de Publicação: 2013
Outros Autores: Ferreira,Dhiego Gomes, Oliveira,Claudio, Almeida,Fernanda Simões de, Shibatta,Oscar Akio, Sofia,Silvia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical ichthyology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
Resumo: Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
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spelling Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplificationBrazilian Eastern basinCatfishEnriched genomic libraryGenetic markerSSRThirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.Sociedade Brasileira de Ictiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581Neotropical Ichthyology v.11 n.3 2013reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/S1679-62252013000300011info:eu-repo/semantics/openAccessSouza-Shibatta,LeniceFerreira,Dhiego GomesOliveira,ClaudioAlmeida,Fernanda Simões deShibatta,Oscar AkioSofia,Silvia Helenaeng2015-10-09T00:00:00Zoai:scielo:S1679-62252013000300581Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2015-10-09T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false
dc.title.none.fl_str_mv Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
spellingShingle Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
Souza-Shibatta,Lenice
Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
title_short Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_full Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_fullStr Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_full_unstemmed Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_sort Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
author Souza-Shibatta,Lenice
author_facet Souza-Shibatta,Lenice
Ferreira,Dhiego Gomes
Oliveira,Claudio
Almeida,Fernanda Simões de
Shibatta,Oscar Akio
Sofia,Silvia Helena
author_role author
author2 Ferreira,Dhiego Gomes
Oliveira,Claudio
Almeida,Fernanda Simões de
Shibatta,Oscar Akio
Sofia,Silvia Helena
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Souza-Shibatta,Lenice
Ferreira,Dhiego Gomes
Oliveira,Claudio
Almeida,Fernanda Simões de
Shibatta,Oscar Akio
Sofia,Silvia Helena
dc.subject.por.fl_str_mv Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
topic Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
description Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300581
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1679-62252013000300011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
dc.source.none.fl_str_mv Neotropical Ichthyology v.11 n.3 2013
reponame:Neotropical ichthyology (Online)
instname:Sociedade Brasileira de Ictiologia (SBI)
instacron:SBI
instname_str Sociedade Brasileira de Ictiologia (SBI)
instacron_str SBI
institution SBI
reponame_str Neotropical ichthyology (Online)
collection Neotropical ichthyology (Online)
repository.name.fl_str_mv Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)
repository.mail.fl_str_mv ||neoichth@nupelia.uem.br
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