Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification

Detalhes bibliográficos
Autor(a) principal: Souza-shibatta, Lenice
Data de Publicação: 2013
Outros Autores: Ferreira, Dhiego Gomes, Oliveira, Claudio [UNESP], Almeida, Fernanda Simões De, Shibatta, Oscar Akio, Sofia, Silvia Helena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1679-62252013000300011
http://hdl.handle.net/11449/211889
Resumo: Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
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spelling Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplificationBrazilian Eastern basinCatfishEnriched genomic libraryGenetic markerSSRThirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.Treze loci microssatélites foram isolados e caracterizados em Microglanis cottoides. Destes, dois foram monomórficos e 11 foram polimórficos. Estes loci polimórficos foram testados em 24 indivíduos de uma população selvagem e produziram um total de 108 alelos diferentes, com níveis de variabilidade alta, variando de 2 a 20, com uma média de 8,3 alelos por locus. A heterozigosidade observada e esperada variou de 0,125 a 0,958 e 0,119 a 0,931, respectivamente. Um elevado valor de exclusão de paternidade e um baixo valor de identidade genética foram obtidos, demostrando que o conjunto de loci descritos no presente trabalho exibe boa aplicabilidade no estudo de parentesco e diferenciação populacional em M. cottoides. Adicionalmente, os treze primers de microssatélites desenvolvidos para M. cottoides foram testados em outras quatro espécies de Pseudopimelodidae e a transferabilidade foi obtida para a maioria dos loci.Universidade Estadual de Londrina, Programa de Pós-Graduação em Ciências BiológicasUniversidade Estadual de Londrina, Programa de Pós-Graduação em Genética e Biologia MolecularUniversidade Paulista Júlio Mesquita Filho, Instituto de BiociênciasUniversidade Paulista Júlio Mesquita Filho, Instituto de BiociênciasSociedade Brasileira de IctiologiaUniversidade Estadual de LondrinaUniversidade Estadual Paulista (Unesp)Souza-shibatta, LeniceFerreira, Dhiego GomesOliveira, Claudio [UNESP]Almeida, Fernanda Simões DeShibatta, Oscar AkioSofia, Silvia Helena2021-07-14T10:31:12Z2021-07-14T10:31:12Z2013-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article581-585application/pdfhttp://dx.doi.org/10.1590/S1679-62252013000300011Neotropical Ichthyology. Sociedade Brasileira de Ictiologia, v. 11, n. 03, p. 581-585, 2013.1679-62251982-0224http://hdl.handle.net/11449/21188910.1590/S1679-62252013000300011S1679-62252013000300581S1679-62252013000300581.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeotropical Ichthyologyinfo:eu-repo/semantics/openAccess2023-12-14T06:18:05Zoai:repositorio.unesp.br:11449/211889Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:17:06.880339Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
spellingShingle Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
Souza-shibatta, Lenice
Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
title_short Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_full Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_fullStr Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_full_unstemmed Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
title_sort Development and characterization of microsatellite loci of Microglanis cottoides (Siluriformes: Pseudopimelodidae) and cross-species amplification
author Souza-shibatta, Lenice
author_facet Souza-shibatta, Lenice
Ferreira, Dhiego Gomes
Oliveira, Claudio [UNESP]
Almeida, Fernanda Simões De
Shibatta, Oscar Akio
Sofia, Silvia Helena
author_role author
author2 Ferreira, Dhiego Gomes
Oliveira, Claudio [UNESP]
Almeida, Fernanda Simões De
Shibatta, Oscar Akio
Sofia, Silvia Helena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Souza-shibatta, Lenice
Ferreira, Dhiego Gomes
Oliveira, Claudio [UNESP]
Almeida, Fernanda Simões De
Shibatta, Oscar Akio
Sofia, Silvia Helena
dc.subject.por.fl_str_mv Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
topic Brazilian Eastern basin
Catfish
Enriched genomic library
Genetic marker
SSR
description Thirteen microsatellite loci were isolated and characterized in Microglanis cottoides. Of these, two were monomorphic and 11 were polymorphic. These polymorphic loci tested on 24 individuals from a wild population produced a total of 108 different alleles, with levels of variability high, ranging from 2 to 20, with an average of 8.3 alleles per locus. The observed and expected heterozygosity ranged from 0.125 to 0.958 and from 0.119 to 0.931, respectively. A high combined probability of paternity exclusion value and a low probability combined genetic identity value obtained show that the set of loci described herein displays good suitability for paternity studies and differentiation of M. cottoides. Additionally, all thirteen microsatellite primers developed for M. cottoides were tested in four other Pseudopimelodidae species and successful cross-species amplification was achieved for the majority of loci.
publishDate 2013
dc.date.none.fl_str_mv 2013-09
2021-07-14T10:31:12Z
2021-07-14T10:31:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1679-62252013000300011
Neotropical Ichthyology. Sociedade Brasileira de Ictiologia, v. 11, n. 03, p. 581-585, 2013.
1679-6225
1982-0224
http://hdl.handle.net/11449/211889
10.1590/S1679-62252013000300011
S1679-62252013000300581
S1679-62252013000300581.pdf
url http://dx.doi.org/10.1590/S1679-62252013000300011
http://hdl.handle.net/11449/211889
identifier_str_mv Neotropical Ichthyology. Sociedade Brasileira de Ictiologia, v. 11, n. 03, p. 581-585, 2013.
1679-6225
1982-0224
10.1590/S1679-62252013000300011
S1679-62252013000300581
S1679-62252013000300581.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neotropical Ichthyology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 581-585
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129183472156672

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