Development and validation of SSR markers for Coffea arabica L.

Detalhes bibliográficos
Autor(a) principal: MISSIO, R. F.
Data de Publicação: 2009
Outros Autores: CAIXETA, E. T., ZAMBOLIM, E. M., ZAMBOLIM, L., SAKIYAMA, N. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403
Resumo: With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.
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spelling Development and validation of SSR markers for Coffea arabica L.SSR markerCoffeeEnriched genomic libraryMolecular markerWith the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.ROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA.MISSIO, R. F.CAIXETA, E. T.ZAMBOLIM, E. M.ZAMBOLIM, L.SAKIYAMA, N. S.2011-04-09T22:27:59Z2011-04-09T22:27:59Z2011-03-1020092011-04-10T11:11:11Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleIn.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009.http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2017-08-15T22:10:23Zoai:www.alice.cnptia.embrapa.br:doc/880403Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542017-08-15T22:10:23falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542017-08-15T22:10:23Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Development and validation of SSR markers for Coffea arabica L.
title Development and validation of SSR markers for Coffea arabica L.
spellingShingle Development and validation of SSR markers for Coffea arabica L.
MISSIO, R. F.
SSR marker
Coffee
Enriched genomic library
Molecular marker
title_short Development and validation of SSR markers for Coffea arabica L.
title_full Development and validation of SSR markers for Coffea arabica L.
title_fullStr Development and validation of SSR markers for Coffea arabica L.
title_full_unstemmed Development and validation of SSR markers for Coffea arabica L.
title_sort Development and validation of SSR markers for Coffea arabica L.
author MISSIO, R. F.
author_facet MISSIO, R. F.
CAIXETA, E. T.
ZAMBOLIM, E. M.
ZAMBOLIM, L.
SAKIYAMA, N. S.
author_role author
author2 CAIXETA, E. T.
ZAMBOLIM, E. M.
ZAMBOLIM, L.
SAKIYAMA, N. S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv ROBSON FERNANDO MISSIO, UNIVERSIDADE FEDERAL DE VIÇOSA; EVELINE TEIXEIRA CAIXETA, SAPC; EUNIZE MACIEL ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; LAÉRCIO ZAMBOLIM, UNIVERSIDADE FEDERAL DE VIÇOSA; NEY SUSSUMU SAKIYAMA, UNIVERSIDADE FEDERAL DE VIÇOSA.
dc.contributor.author.fl_str_mv MISSIO, R. F.
CAIXETA, E. T.
ZAMBOLIM, E. M.
ZAMBOLIM, L.
SAKIYAMA, N. S.
dc.subject.por.fl_str_mv SSR marker
Coffee
Enriched genomic library
Molecular marker
topic SSR marker
Coffee
Enriched genomic library
Molecular marker
description With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)15 and (AGG)10 were constructed. A total of 835 clones were sequenced and 756 presented good quality sequences. Redundant sequences were observed for 113 clones (14.94%). SSRs were found in 287 clones (38%). An estimated size of 417.5Kb of the C. arabica genome was sampled, with an average of one SSR per 1.46Kb. Dinucleotide repeats were more frequent than trinucleotides. Four repeat sequences, (AG/CT)n, (AC/GT)n, (AAG/CTT)n, and (AGG/CCT)n represented 61.1% of the total observed. A total of 96 SSR primers were designed and tested by PCR for two C. arabica genotypes. Ninety new SSR markers were validated for further genetic studies of C. arabica.
publishDate 2009
dc.date.none.fl_str_mv 2009
2011-04-09T22:27:59Z
2011-04-09T22:27:59Z
2011-03-10
2011-04-10T11:11:11Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv In.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403
identifier_str_mv In.: CROP BREEDING AND APPLIED BIOTECHNOLOGY. v.9, p.361-371, 2009.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/880403
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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