Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015 |
Resumo: | GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition. |
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Brazilian Journal of Microbiology |
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Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industryBacillus alkalophilic CGIICGTasebeta-cyclodextrinstarch hydrolysisenzyme activityGCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition.Sociedade Brasileira de Microbiologia2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015Brazilian Journal of Microbiology v.35 n.3 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000200015info:eu-repo/semantics/openAccessFreitas,Telma Luisa deMonti,RubensContiero,Jonaseng2005-11-25T00:00:00Zoai:scielo:S1517-83822004000200015Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-11-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
spellingShingle |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry Freitas,Telma Luisa de Bacillus alkalophilic CGII CGTase beta-cyclodextrin starch hydrolysis enzyme activity |
title_short |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_full |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_fullStr |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_full_unstemmed |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
title_sort |
Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry |
author |
Freitas,Telma Luisa de |
author_facet |
Freitas,Telma Luisa de Monti,Rubens Contiero,Jonas |
author_role |
author |
author2 |
Monti,Rubens Contiero,Jonas |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Freitas,Telma Luisa de Monti,Rubens Contiero,Jonas |
dc.subject.por.fl_str_mv |
Bacillus alkalophilic CGII CGTase beta-cyclodextrin starch hydrolysis enzyme activity |
topic |
Bacillus alkalophilic CGII CGTase beta-cyclodextrin starch hydrolysis enzyme activity |
description |
GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822004000200015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.35 n.3 2004 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200127700992 |