Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036 |
Resumo: | The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection. |
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Brazilian Journal of Microbiology |
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spelling |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategyHBVPreS1ProteomicsGRP75The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection.Sociedade Brasileira de Microbiologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036Brazilian Journal of Microbiology v.41 n.2 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000200036info:eu-repo/semantics/openAccessCui,LunbiaoGe,YiyueQi,YuhuaShi,ZhiyangJiao,YongjunQi,XianZhai,XiangjunWang,Huaeng2010-04-16T00:00:00Zoai:scielo:S1517-83822010000200036Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-04-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
title |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
spellingShingle |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy Cui,Lunbiao HBV PreS1 Proteomics GRP75 |
title_short |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
title_full |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
title_fullStr |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
title_full_unstemmed |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
title_sort |
Identification of GRP75 as a novel PreS1 binding protein using a proteomics strategy |
author |
Cui,Lunbiao |
author_facet |
Cui,Lunbiao Ge,Yiyue Qi,Yuhua Shi,Zhiyang Jiao,Yongjun Qi,Xian Zhai,Xiangjun Wang,Hua |
author_role |
author |
author2 |
Ge,Yiyue Qi,Yuhua Shi,Zhiyang Jiao,Yongjun Qi,Xian Zhai,Xiangjun Wang,Hua |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cui,Lunbiao Ge,Yiyue Qi,Yuhua Shi,Zhiyang Jiao,Yongjun Qi,Xian Zhai,Xiangjun Wang,Hua |
dc.subject.por.fl_str_mv |
HBV PreS1 Proteomics GRP75 |
topic |
HBV PreS1 Proteomics GRP75 |
description |
The PreS1 region of the L protein is important in cell attachment and consequently in hepatitis B virus (HBV) infectivity. To identify novel PreS1 interacting protein, we performed Glutathione-S-transferase (GST) pull-down, two-dimensional gel electrophoresis (2-DE) and mass spectrometry assays. Glucose-regulated proteins (GRP) 78 and 75 were found to bind PreS1. The interactions between PreS1 and GRP75 were confirmed by a co-immunoprecipitation experiment. GRP78 and GRP75 may play important roles in mediating HBV virion entering into hepatocyte and regulating proper folding of the L protein due to their critical functions in protein folding and trafficking. The finding of novel PreS1 binding protein enriches our knowledge about molecular mechanism of HBV infection. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822010000200036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.41 n.2 2010 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203028062208 |