Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027 |
Resumo: | In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-. |
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Brazilian Journal of Microbiology |
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Evaluation of an indirect ELISA for the detection of Salmonella in chicken meatSalmonellamonoclonal antibodypoliclonal antibodyELISAIn this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-.Sociedade Brasileira de Microbiologia2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027Brazilian Journal of Microbiology v.37 n.3 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000300027info:eu-repo/semantics/openAccessSchneid,Andréa dos SantosRodrigues,Kelly LameiroChemello,DaviTondo,Eduardo CesarAyub,Marco Antônio ZacchiaAleixo,José Antonio Guimarãeseng2006-12-18T00:00:00Zoai:scielo:S1517-83822006000300027Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-12-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
title |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
spellingShingle |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat Schneid,Andréa dos Santos Salmonella monoclonal antibody policlonal antibody ELISA |
title_short |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
title_full |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
title_fullStr |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
title_full_unstemmed |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
title_sort |
Evaluation of an indirect ELISA for the detection of Salmonella in chicken meat |
author |
Schneid,Andréa dos Santos |
author_facet |
Schneid,Andréa dos Santos Rodrigues,Kelly Lameiro Chemello,Davi Tondo,Eduardo Cesar Ayub,Marco Antônio Zacchia Aleixo,José Antonio Guimarães |
author_role |
author |
author2 |
Rodrigues,Kelly Lameiro Chemello,Davi Tondo,Eduardo Cesar Ayub,Marco Antônio Zacchia Aleixo,José Antonio Guimarães |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Schneid,Andréa dos Santos Rodrigues,Kelly Lameiro Chemello,Davi Tondo,Eduardo Cesar Ayub,Marco Antônio Zacchia Aleixo,José Antonio Guimarães |
dc.subject.por.fl_str_mv |
Salmonella monoclonal antibody policlonal antibody ELISA |
topic |
Salmonella monoclonal antibody policlonal antibody ELISA |
description |
In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonellaenterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7:-:-. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822006000300027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.37 n.3 2006 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122200634163200 |