Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat

Detalhes bibliográficos
Autor(a) principal: Schneid, Andréa dos Santos
Data de Publicação: 2006
Outros Autores: Rodrigues, Kelly Lameiro, Chemello, Davi, Tondo, Eduardo Cesar, Ayub, Marco Antônio Záchia, Aleixo, José Antônio Guimarães
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/20395
Resumo: In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonella enterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7
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spelling Schneid, Andréa dos SantosRodrigues, Kelly LameiroChemello, DaviTondo, Eduardo CesarAyub, Marco Antônio ZáchiaAleixo, José Antônio Guimarães2010-04-16T09:14:44Z20061517-8382http://hdl.handle.net/10183/20395000601373In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonella enterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7application/pdfengBrazilian journal of microbiology. São Paulo. vol. 37, n. 3 (jul/set. 2006), p. 350-355SalmonellaCarne de frangoAnticorpo monoclonalELISASalmonellaMonoclonal antibodyPoliclonal antibodyELISAEvaluation of an indirect Elisa for the detection of Salmonella in chicken meatinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000601373.pdf000601373.pdfTexto completo (inglês)application/pdf145396http://www.lume.ufrgs.br/bitstream/10183/20395/1/000601373.pdf5c7ed5a370a562bb5ed926d6ca6c3003MD51TEXT000601373.pdf.txt000601373.pdf.txtExtracted Texttext/plain26565http://www.lume.ufrgs.br/bitstream/10183/20395/2/000601373.pdf.txtf93afaf6c1d3630108ce3570ed4dfe90MD52THUMBNAIL000601373.pdf.jpg000601373.pdf.jpgGenerated Thumbnailimage/jpeg1750http://www.lume.ufrgs.br/bitstream/10183/20395/3/000601373.pdf.jpg9f2335f6efe3f737851b966a594f918cMD5310183/203952018-10-08 08:34:13.121oai:www.lume.ufrgs.br:10183/20395Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-08T11:34:13Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
title Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
spellingShingle Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
Schneid, Andréa dos Santos
Salmonella
Carne de frango
Anticorpo monoclonal
ELISA
Salmonella
Monoclonal antibody
Policlonal antibody
ELISA
title_short Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
title_full Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
title_fullStr Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
title_full_unstemmed Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
title_sort Evaluation of an indirect Elisa for the detection of Salmonella in chicken meat
author Schneid, Andréa dos Santos
author_facet Schneid, Andréa dos Santos
Rodrigues, Kelly Lameiro
Chemello, Davi
Tondo, Eduardo Cesar
Ayub, Marco Antônio Záchia
Aleixo, José Antônio Guimarães
author_role author
author2 Rodrigues, Kelly Lameiro
Chemello, Davi
Tondo, Eduardo Cesar
Ayub, Marco Antônio Záchia
Aleixo, José Antônio Guimarães
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Schneid, Andréa dos Santos
Rodrigues, Kelly Lameiro
Chemello, Davi
Tondo, Eduardo Cesar
Ayub, Marco Antônio Záchia
Aleixo, José Antônio Guimarães
dc.subject.por.fl_str_mv Salmonella
Carne de frango
Anticorpo monoclonal
ELISA
topic Salmonella
Carne de frango
Anticorpo monoclonal
ELISA
Salmonella
Monoclonal antibody
Policlonal antibody
ELISA
dc.subject.eng.fl_str_mv Salmonella
Monoclonal antibody
Policlonal antibody
ELISA
description In this work, an indirect ELISA based on a monoclonal antibody (MAb) specific for an outer membrane protein of Salmonella enterica serovar Enteritidis was used for detection of Salmonella in 154 samples of chicken meat. Its efficiency was determined through comparison with the results obtained from the conventional method. The prevalence of samples contaminated with Salmonella was 23% with the conventional culture method, and 26% with the ELISA. From thirty-five samples positive for Salmonella by the conventional method, 33 were also positive by ELISA. Seven other samples were only positive in the ELISA. Comparison of the results obtained in the two methods showed an ELISA sensitivity and specificity of 94%, and positive and negative predictive values of 82% and 98% respectively. The serotyping of the isolates revealed 31 Salmonella enterica serovar Enteritidis, 2 Salmonella enterica serovar Heidelberg, 1 Salmonella enterica serovar Choleraesuis and 1 Salmonella enterica sorovar 6,7
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2010-04-16T09:14:44Z
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/20395
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.nrb.pt_BR.fl_str_mv 000601373
identifier_str_mv 1517-8382
000601373
url http://hdl.handle.net/10183/20395
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of microbiology. São Paulo. vol. 37, n. 3 (jul/set. 2006), p. 350-355
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