Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377 |
Resumo: | Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments. |
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Brazilian Journal of Microbiology |
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Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortiumAlcanivoraxPseudomonasRhodococcusbioremediationbioaugmentationThree bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments.Sociedade Brasileira de Microbiologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377Brazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246120131276info:eu-repo/semantics/openAccessSantisi,SantinaCappello,SimoneCatalfamo,MaurizioMancini,GiuseppeHassanshahian,MehdiGenovese,LucreziaGiuliano,LauraYakimov,Michail M.eng2015-11-27T00:00:00Zoai:scielo:S1517-83822015000200377Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-11-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
title |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
spellingShingle |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium Santisi,Santina Alcanivorax Pseudomonas Rhodococcus bioremediation bioaugmentation |
title_short |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
title_full |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
title_fullStr |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
title_full_unstemmed |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
title_sort |
Biodegradation of crude oil by individual bacterial strains and a mixed bacterial consortium |
author |
Santisi,Santina |
author_facet |
Santisi,Santina Cappello,Simone Catalfamo,Maurizio Mancini,Giuseppe Hassanshahian,Mehdi Genovese,Lucrezia Giuliano,Laura Yakimov,Michail M. |
author_role |
author |
author2 |
Cappello,Simone Catalfamo,Maurizio Mancini,Giuseppe Hassanshahian,Mehdi Genovese,Lucrezia Giuliano,Laura Yakimov,Michail M. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santisi,Santina Cappello,Simone Catalfamo,Maurizio Mancini,Giuseppe Hassanshahian,Mehdi Genovese,Lucrezia Giuliano,Laura Yakimov,Michail M. |
dc.subject.por.fl_str_mv |
Alcanivorax Pseudomonas Rhodococcus bioremediation bioaugmentation |
topic |
Alcanivorax Pseudomonas Rhodococcus bioremediation bioaugmentation |
description |
Three bacterial isolates identified as Alcanivorax borkumensis SK2, Rhodococcus erythropolis HS4 and Pseudomonas stutzeri SDM, based on 16S rRNA gene sequences, were isolated from crude oil enrichments of natural seawater. Single strains and four bacterial consortia designed by mixing the single bacterial cultures respectively in the following ratios: (Alcanivorax: Pseudomonas, 1:1), (Alcanivorax: Rhodococcus, 1:1), (Pseudomonas: Rhodococcus, 1:1), and (Alcanivorax: Pseudomonas: Rhodococcus, 1:1:1), were analyzed in order to evaluate their oil degrading capability. All experiments were carried out in microcosms systems containing seawater (with and without addition of inorganic nutrients) and crude oil (unique carbon source). Measures of total and live bacterial abundance, Card-FISH and quali-, quantitative analysis of hydrocarbons (GC-FID) were carried out in order to elucidate the co-operative action of mixed microbial populations in the process of biodegradation of crude oil. All data obtained confirmed the fundamental role of bacteria belonging to Alcanivorax genus in the degradation of linear hydrocarbons in oil polluted environments. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200377 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-838246120131276 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.46 n.2 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207437324288 |