Occurrence of aflatoxin B1 in natural products

Detalhes bibliográficos
Autor(a) principal: Prado,Guilherme
Data de Publicação: 2012
Outros Autores: Altoé,Aline F., Gomes,Tatiana C. B., Leal,Alexandre S., Morais,Vanessa A. D., Oliveira,Marize S., Ferreira,Marli B., Gomes,Mateus B., Paschoal,Fabiano N., Souza,Rafael von S., Silva,Daniela A., Madeira,Jovita E. G. Cruz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026
Resumo: The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on extraction through acetone:water (85:15), immunoaffinity column purification followed by separation and detection in high efficiency liquid chromatography. Under the conditions of analysis, the Limits of Detection and Quantification were 0.6 µg kg-1 and 1.0 µg kg-1respectively. The complete sets of analyses were carried out in duplicate. Aflatoxin B1 was noticed in a single sample (< 1.0 µg kg-1). The results revealed low aflatoxin B1contamination in the products under analysis. However, it is required to establish a broad monitoring program in order to obtain additional data and check up on the actual extension of contamination.
id SBM-1_2ab363e629dc9f9e43f70f0d71ef5c4b
oai_identifier_str oai:scielo:S1517-83822012000400026
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling Occurrence of aflatoxin B1 in natural productsmedicinal plantsfungimycotoxinhigh-performance liquid chromatographyHPLCThe media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on extraction through acetone:water (85:15), immunoaffinity column purification followed by separation and detection in high efficiency liquid chromatography. Under the conditions of analysis, the Limits of Detection and Quantification were 0.6 µg kg-1 and 1.0 µg kg-1respectively. The complete sets of analyses were carried out in duplicate. Aflatoxin B1 was noticed in a single sample (< 1.0 µg kg-1). The results revealed low aflatoxin B1contamination in the products under analysis. However, it is required to establish a broad monitoring program in order to obtain additional data and check up on the actual extension of contamination.Sociedade Brasileira de Microbiologia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026Brazilian Journal of Microbiology v.43 n.4 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000400026info:eu-repo/semantics/openAccessPrado,GuilhermeAltoé,Aline F.Gomes,Tatiana C. B.Leal,Alexandre S.Morais,Vanessa A. D.Oliveira,Marize S.Ferreira,Marli B.Gomes,Mateus B.Paschoal,Fabiano N.Souza,Rafael von S.Silva,Daniela A.Madeira,Jovita E. G. Cruzeng2013-02-19T00:00:00Zoai:scielo:S1517-83822012000400026Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2013-02-19T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Occurrence of aflatoxin B1 in natural products
title Occurrence of aflatoxin B1 in natural products
spellingShingle Occurrence of aflatoxin B1 in natural products
Prado,Guilherme
medicinal plants
fungi
mycotoxin
high-performance liquid chromatography
HPLC
title_short Occurrence of aflatoxin B1 in natural products
title_full Occurrence of aflatoxin B1 in natural products
title_fullStr Occurrence of aflatoxin B1 in natural products
title_full_unstemmed Occurrence of aflatoxin B1 in natural products
title_sort Occurrence of aflatoxin B1 in natural products
author Prado,Guilherme
author_facet Prado,Guilherme
Altoé,Aline F.
Gomes,Tatiana C. B.
Leal,Alexandre S.
Morais,Vanessa A. D.
Oliveira,Marize S.
Ferreira,Marli B.
Gomes,Mateus B.
Paschoal,Fabiano N.
Souza,Rafael von S.
Silva,Daniela A.
Madeira,Jovita E. G. Cruz
author_role author
author2 Altoé,Aline F.
Gomes,Tatiana C. B.
Leal,Alexandre S.
Morais,Vanessa A. D.
Oliveira,Marize S.
Ferreira,Marli B.
Gomes,Mateus B.
Paschoal,Fabiano N.
Souza,Rafael von S.
Silva,Daniela A.
Madeira,Jovita E. G. Cruz
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Prado,Guilherme
Altoé,Aline F.
Gomes,Tatiana C. B.
Leal,Alexandre S.
Morais,Vanessa A. D.
Oliveira,Marize S.
Ferreira,Marli B.
Gomes,Mateus B.
Paschoal,Fabiano N.
Souza,Rafael von S.
Silva,Daniela A.
Madeira,Jovita E. G. Cruz
dc.subject.por.fl_str_mv medicinal plants
fungi
mycotoxin
high-performance liquid chromatography
HPLC
topic medicinal plants
fungi
mycotoxin
high-performance liquid chromatography
HPLC
description The media claims for the consumption of natural resource-based food have gradually increased in both developing and developed countries. The interest in the safety of these products is partially due to the possible presence of toxigenic fungi acting as mycotoxin producers, such as aflatoxins produced during the secondary metabolism of Aspergillus flavus, A. parasiticus and A. nomius. Aflatoxins, mainly aflatoxin B1, are directly associated with liver cancer in human beings. This paper is aimed at evaluating the presence of aflatoxin B1 in a few vegetable drugs, dried plant extracts and industrialized products traded in 2010 in the city of Belo Horizonte, State of Minas Gerais, Brazil. The method used for the quantification of aflatoxin B1 was based on extraction through acetone:water (85:15), immunoaffinity column purification followed by separation and detection in high efficiency liquid chromatography. Under the conditions of analysis, the Limits of Detection and Quantification were 0.6 µg kg-1 and 1.0 µg kg-1respectively. The complete sets of analyses were carried out in duplicate. Aflatoxin B1 was noticed in a single sample (< 1.0 µg kg-1). The results revealed low aflatoxin B1contamination in the products under analysis. However, it is required to establish a broad monitoring program in order to obtain additional data and check up on the actual extension of contamination.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000400026
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.4 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122204811689984