Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)

Detalhes bibliográficos
Autor(a) principal: Rulík,Martin
Data de Publicação: 2014
Outros Autores: Chaudhary,Prem Prashant
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016
Resumo: Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in this particular geographical region. However more detailed and specific studies are warranted in order to properly assess the community structure of MTB's in fresh water sediments.
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spelling Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)magnetotactic bacteriasedimentPCRcloningphylogeny16S rRNA geneMagnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in this particular geographical region. However more detailed and specific studies are warranted in order to properly assess the community structure of MTB's in fresh water sediments.Sociedade Brasileira de Microbiologia2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016Brazilian Journal of Microbiology v.45 n.4 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000400016info:eu-repo/semantics/openAccessRulík,MartinChaudhary,Prem Prashanteng2015-02-13T00:00:00Zoai:scielo:S1517-83822014000400016Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-02-13T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
title Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
spellingShingle Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
Rulík,Martin
magnetotactic bacteria
sediment
PCR
cloning
phylogeny
16S rRNA gene
title_short Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
title_full Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
title_fullStr Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
title_full_unstemmed Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
title_sort Molecular identification of the occurrence of magnetotactic bacteria in fresh water sediments (Czech Republic)
author Rulík,Martin
author_facet Rulík,Martin
Chaudhary,Prem Prashant
author_role author
author2 Chaudhary,Prem Prashant
author2_role author
dc.contributor.author.fl_str_mv Rulík,Martin
Chaudhary,Prem Prashant
dc.subject.por.fl_str_mv magnetotactic bacteria
sediment
PCR
cloning
phylogeny
16S rRNA gene
topic magnetotactic bacteria
sediment
PCR
cloning
phylogeny
16S rRNA gene
description Magnetotactic bacteria (MTB) are of considerable interest because of their importance in the manufacture of various bioinspired materials. In order to find out the status of magnetotactic bacteria at three different sediment in Czech Republic, samples collected from both standing and running freshwaters were subjected to molecular diversity analysis by using 16S rRNA gene approach. Total community DNA from sediment sample was isolated and used for PCR, cloning and sequence analysis. Of the 24 analyzed sequences, six clones are closely related to Magnetobacterium sp. affiliated with the Nitrospira phylum which showed the dominance of Magnetobacterium phylotypes in the sample. This study will provide useful insight about the community structure of MTB in this particular geographical region. However more detailed and specific studies are warranted in order to properly assess the community structure of MTB's in fresh water sediments.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000400016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.4 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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