Simple homemade apparatus for harvesting uncultured magnetotactic microorganisms

• Autor(a) principal: Lins,Ulysses
• Data de Publicação: 2003
• Outros Autores: Freitas,Flavia, Keim,Carolina Neumann, Barros,Henrique Lins de, Esquivel,Darci Motta S., Farina,Marcos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200004
• topic: Magnetotactic bacteria; harvesting uncultured bacteria; electron microscopy

A simple apparatus for harvesting uncultured magnetotactic microorganisms is described. This apparatus consists of a glass container with two openings. A large opening on the topside is used to introduce the sediment and water. The sediment and water are previously stored in loosely capped bottles previously tested for the presence of magnetotactic bacteria. The apparatus is exposed to a properly aligned magnetic field of a homemade coil and the bacteria are removed through the capillary end of the second opening of the container. Harvested bacteria can then be used to ultrastrucutral studies using electron spectroscopic imaging. Large numbers of magnetotactic bacteria consisting of cocci and rod-shaped cells were efficiently collected from different environments. This apparatus is useful for microbiological studies on uncultured magnetotactic bacteria, especially in molecular approaches for phylogenetic investigations that give information on the natural diversity of microbial communities.