16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting

Detalhes bibliográficos
Autor(a) principal: Navarro-Noya,Yendi
Data de Publicação: 2012
Outros Autores: Hernández-Rodríguez,César, Zenteno,Juan C, Buentello-Volante,Beatriz, Cancino-Díaz,Mario E, Jan-Roblero,Janet, Cancino-Díaz,Juan C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
Resumo: Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
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spelling 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprintingendophthalmitisStaphylococcus epidermidisPCRDGGEConventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.Sociedade Brasileira de Microbiologia2012-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033Brazilian Journal of Microbiology v.43 n.1 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000100033info:eu-repo/semantics/openAccessNavarro-Noya,YendiHernández-Rodríguez,CésarZenteno,Juan CBuentello-Volante,BeatrizCancino-Díaz,Mario EJan-Roblero,JanetCancino-Díaz,Juan Ceng2012-05-02T00:00:00Zoai:scielo:S1517-83822012000100033Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2012-05-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
spellingShingle 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
Navarro-Noya,Yendi
endophthalmitis
Staphylococcus epidermidis
PCR
DGGE
title_short 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_full 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_fullStr 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_full_unstemmed 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
title_sort 16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR- Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting
author Navarro-Noya,Yendi
author_facet Navarro-Noya,Yendi
Hernández-Rodríguez,César
Zenteno,Juan C
Buentello-Volante,Beatriz
Cancino-Díaz,Mario E
Jan-Roblero,Janet
Cancino-Díaz,Juan C
author_role author
author2 Hernández-Rodríguez,César
Zenteno,Juan C
Buentello-Volante,Beatriz
Cancino-Díaz,Mario E
Jan-Roblero,Janet
Cancino-Díaz,Juan C
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Navarro-Noya,Yendi
Hernández-Rodríguez,César
Zenteno,Juan C
Buentello-Volante,Beatriz
Cancino-Díaz,Mario E
Jan-Roblero,Janet
Cancino-Díaz,Juan C
dc.subject.por.fl_str_mv endophthalmitis
Staphylococcus epidermidis
PCR
DGGE
topic endophthalmitis
Staphylococcus epidermidis
PCR
DGGE
description Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.
publishDate 2012
dc.date.none.fl_str_mv 2012-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822012000100033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.43 n.1 2012
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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