Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Vaucher,Rodrigo de Almeida
Data de Publicação: 2011
Outros Autores: Dezen,Diogenes, Simonetti,Amauri Braga, Spilki,Fernando Rosado, Roehe,Paulo Michel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029
Resumo: Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
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spelling Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from BrazilBovine parainfluenza virus type 3bPIV-3phylogenetic analysisGenomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.Sociedade Brasileira de Microbiologia2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029Brazilian Journal of Microbiology v.42 n.4 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000400029info:eu-repo/semantics/openAccessVaucher,Rodrigo de AlmeidaDezen,DiogenesSimonetti,Amauri BragaSpilki,Fernando RosadoRoehe,Paulo Micheleng2015-06-30T00:00:00Zoai:scielo:S1517-83822011000400029Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
spellingShingle Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Vaucher,Rodrigo de Almeida
Bovine parainfluenza virus type 3
bPIV-3
phylogenetic analysis
title_short Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_fullStr Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full_unstemmed Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_sort Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
author Vaucher,Rodrigo de Almeida
author_facet Vaucher,Rodrigo de Almeida
Dezen,Diogenes
Simonetti,Amauri Braga
Spilki,Fernando Rosado
Roehe,Paulo Michel
author_role author
author2 Dezen,Diogenes
Simonetti,Amauri Braga
Spilki,Fernando Rosado
Roehe,Paulo Michel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vaucher,Rodrigo de Almeida
Dezen,Diogenes
Simonetti,Amauri Braga
Spilki,Fernando Rosado
Roehe,Paulo Michel
dc.subject.por.fl_str_mv Bovine parainfluenza virus type 3
bPIV-3
phylogenetic analysis
topic Bovine parainfluenza virus type 3
bPIV-3
phylogenetic analysis
description Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822011000400029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.42 n.4 2011
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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