Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Detalhes bibliográficos
Autor(a) principal: Vaucher, Rodrigo de Almeida
Data de Publicação: 2011
Outros Autores: Dezen, Diogenes, Simonetti, Amauri Braga, Spilki, Fernando Rosado, Roehe, Paulo Michel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/81809
Resumo: Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
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spelling Vaucher, Rodrigo de AlmeidaDezen, DiogenesSimonetti, Amauri BragaSpilki, Fernando RosadoRoehe, Paulo Michel2013-12-03T01:48:52Z20111517-8382http://hdl.handle.net/10183/81809000822590Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.application/pdfengBrazilian journal of microbiology. v. 42, n. 4 (out./dez. 2011), p. 1440-1444Vírus da parainfluenza 3 bovinaVirologiaBovine parainfluenza virus type 3BPIV-3Phylogenetic analysisPhylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazilinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000822590.pdf000822590.pdfTexto completo (inglês)application/pdf176802http://www.lume.ufrgs.br/bitstream/10183/81809/1/000822590.pdf369512563e7bd849ad8f8cde68e1b440MD51TEXT000822590.pdf.txt000822590.pdf.txtExtracted Texttext/plain15299http://www.lume.ufrgs.br/bitstream/10183/81809/2/000822590.pdf.txtf414ddca761241240c44d8f1256d1467MD52THUMBNAIL000822590.pdf.jpg000822590.pdf.jpgGenerated Thumbnailimage/jpeg2104http://www.lume.ufrgs.br/bitstream/10183/81809/3/000822590.pdf.jpgace5df93ac3d2ad9b80ab04cdec6441eMD5310183/818092019-01-11 04:06:51.060494oai:www.lume.ufrgs.br:10183/81809Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-11T06:06:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
spellingShingle Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
Vaucher, Rodrigo de Almeida
Vírus da parainfluenza 3 bovina
Virologia
Bovine parainfluenza virus type 3
BPIV-3
Phylogenetic analysis
title_short Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_fullStr Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_full_unstemmed Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
title_sort Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil
author Vaucher, Rodrigo de Almeida
author_facet Vaucher, Rodrigo de Almeida
Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
author_role author
author2 Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Vaucher, Rodrigo de Almeida
Dezen, Diogenes
Simonetti, Amauri Braga
Spilki, Fernando Rosado
Roehe, Paulo Michel
dc.subject.por.fl_str_mv Vírus da parainfluenza 3 bovina
Virologia
topic Vírus da parainfluenza 3 bovina
Virologia
Bovine parainfluenza virus type 3
BPIV-3
Phylogenetic analysis
dc.subject.eng.fl_str_mv Bovine parainfluenza virus type 3
BPIV-3
Phylogenetic analysis
description Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2013-12-03T01:48:52Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/81809
dc.identifier.issn.pt_BR.fl_str_mv 1517-8382
dc.identifier.nrb.pt_BR.fl_str_mv 000822590
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url http://hdl.handle.net/10183/81809
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of microbiology. v. 42, n. 4 (out./dez. 2011), p. 1440-1444
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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