Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil

Detalhes bibliográficos
Autor(a) principal: Camargo,I. L. B. C.
Data de Publicação: 2008
Outros Autores: Zanella,R. C., Gilmore,M. S., Darini,A. L. C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200014
Resumo: Enterococci are members of commensal flora of animals and insects, but are also important opportunistic pathogens. Our objective was to observe if there was any difference of virulence in several groups of E. faecalis, mainly between vancomycin-resistant E. faecalis (VREFS) of colonization and infection. VREFS and vancomycin-sensitive E. faecalis from Brazil were screened for the presence of virulence factor genes. Phenotypic assays were used to assess in vitro expression, to understand the pathogenic potential of these isolates and to determine whether a correlation exists between virulence and antibiotic resistance. Different virulence profiles were found suggesting that the disseminating clone may have generated several variations. However, our study showed that one constellation of traits appeared most commonly: gelatinase, aggregation substance and esp (GEA). These factors are important because they have been implicated in cell aggregation and biofilm formation. Biofilm formation may promote the conjugation of plasmids harboring resistance and virulence genes, enhancing the probability of entry of new resistance genes into species. Curiously, the profile GEA was not exclusive to VREFS, it was the second most observed in VSEFS isolates from colonization and infection in hospitalized patients and also from rectal swabs of healthy volunteers. Such strains appear to represent the entry gateway to new resistance genes into E. faecalis and may contribute to the spreading of E. faecalis mainly in hospitals.
id SBM-1_77f3f388938262aac1da6a9311baa9f0
oai_identifier_str oai:scielo:S1517-83822008000200014
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from BrazilEnterococcus faecalisVREEspgelatinaseaggregation substanceEnterococci are members of commensal flora of animals and insects, but are also important opportunistic pathogens. Our objective was to observe if there was any difference of virulence in several groups of E. faecalis, mainly between vancomycin-resistant E. faecalis (VREFS) of colonization and infection. VREFS and vancomycin-sensitive E. faecalis from Brazil were screened for the presence of virulence factor genes. Phenotypic assays were used to assess in vitro expression, to understand the pathogenic potential of these isolates and to determine whether a correlation exists between virulence and antibiotic resistance. Different virulence profiles were found suggesting that the disseminating clone may have generated several variations. However, our study showed that one constellation of traits appeared most commonly: gelatinase, aggregation substance and esp (GEA). These factors are important because they have been implicated in cell aggregation and biofilm formation. Biofilm formation may promote the conjugation of plasmids harboring resistance and virulence genes, enhancing the probability of entry of new resistance genes into species. Curiously, the profile GEA was not exclusive to VREFS, it was the second most observed in VSEFS isolates from colonization and infection in hospitalized patients and also from rectal swabs of healthy volunteers. Such strains appear to represent the entry gateway to new resistance genes into E. faecalis and may contribute to the spreading of E. faecalis mainly in hospitals.Sociedade Brasileira de Microbiologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200014Brazilian Journal of Microbiology v.39 n.2 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000200014info:eu-repo/semantics/openAccessCamargo,I. L. B. C.Zanella,R. C.Gilmore,M. S.Darini,A. L. C.eng2008-07-31T00:00:00Zoai:scielo:S1517-83822008000200014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-07-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
title Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
spellingShingle Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
Camargo,I. L. B. C.
Enterococcus faecalis
VRE
Esp
gelatinase
aggregation substance
title_short Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
title_full Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
title_fullStr Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
title_full_unstemmed Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
title_sort Virulence factors in vancomycin-resistant and vancomycin - susceptible Enterococcus faecalis from Brazil
author Camargo,I. L. B. C.
author_facet Camargo,I. L. B. C.
Zanella,R. C.
Gilmore,M. S.
Darini,A. L. C.
author_role author
author2 Zanella,R. C.
Gilmore,M. S.
Darini,A. L. C.
author2_role author
author
author
dc.contributor.author.fl_str_mv Camargo,I. L. B. C.
Zanella,R. C.
Gilmore,M. S.
Darini,A. L. C.
dc.subject.por.fl_str_mv Enterococcus faecalis
VRE
Esp
gelatinase
aggregation substance
topic Enterococcus faecalis
VRE
Esp
gelatinase
aggregation substance
description Enterococci are members of commensal flora of animals and insects, but are also important opportunistic pathogens. Our objective was to observe if there was any difference of virulence in several groups of E. faecalis, mainly between vancomycin-resistant E. faecalis (VREFS) of colonization and infection. VREFS and vancomycin-sensitive E. faecalis from Brazil were screened for the presence of virulence factor genes. Phenotypic assays were used to assess in vitro expression, to understand the pathogenic potential of these isolates and to determine whether a correlation exists between virulence and antibiotic resistance. Different virulence profiles were found suggesting that the disseminating clone may have generated several variations. However, our study showed that one constellation of traits appeared most commonly: gelatinase, aggregation substance and esp (GEA). These factors are important because they have been implicated in cell aggregation and biofilm formation. Biofilm formation may promote the conjugation of plasmids harboring resistance and virulence genes, enhancing the probability of entry of new resistance genes into species. Curiously, the profile GEA was not exclusive to VREFS, it was the second most observed in VSEFS isolates from colonization and infection in hospitalized patients and also from rectal swabs of healthy volunteers. Such strains appear to represent the entry gateway to new resistance genes into E. faecalis and may contribute to the spreading of E. faecalis mainly in hospitals.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822008000200014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.39 n.2 2008
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122201759285248