Comparison of methods for mycobacteria isolation from swine feces

Detalhes bibliográficos
Autor(a) principal: Oliveira,Eugenia Márcia de Deus
Data de Publicação: 2007
Outros Autores: Rodriguez,César Alejandro Rosales, Rocha,Vivianne Cambuí Mesquita, Ambrosio,Simone Rodriguez, Ohara,Patrícia Miyuki, Amaku,Marcos, Ferreira,Fernando, Dias,Ricardo Augusto, Leão,Sylvia Cardoso, Ferreira Neto,José Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019
Resumo: Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).
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spelling Comparison of methods for mycobacteria isolation from swine fecesMycobacterium aviumisolationfecesswineSwine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).Sociedade Brasileira de Microbiologia2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019Brazilian Journal of Microbiology v.38 n.4 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000400019info:eu-repo/semantics/openAccessOliveira,Eugenia Márcia de DeusRodriguez,César Alejandro RosalesRocha,Vivianne Cambuí MesquitaAmbrosio,Simone RodriguezOhara,Patrícia MiyukiAmaku,MarcosFerreira,FernandoDias,Ricardo AugustoLeão,Sylvia CardosoFerreira Neto,José Soareseng2008-01-28T00:00:00Zoai:scielo:S1517-83822007000400019Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-01-28T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Comparison of methods for mycobacteria isolation from swine feces
title Comparison of methods for mycobacteria isolation from swine feces
spellingShingle Comparison of methods for mycobacteria isolation from swine feces
Oliveira,Eugenia Márcia de Deus
Mycobacterium avium
isolation
feces
swine
title_short Comparison of methods for mycobacteria isolation from swine feces
title_full Comparison of methods for mycobacteria isolation from swine feces
title_fullStr Comparison of methods for mycobacteria isolation from swine feces
title_full_unstemmed Comparison of methods for mycobacteria isolation from swine feces
title_sort Comparison of methods for mycobacteria isolation from swine feces
author Oliveira,Eugenia Márcia de Deus
author_facet Oliveira,Eugenia Márcia de Deus
Rodriguez,César Alejandro Rosales
Rocha,Vivianne Cambuí Mesquita
Ambrosio,Simone Rodriguez
Ohara,Patrícia Miyuki
Amaku,Marcos
Ferreira,Fernando
Dias,Ricardo Augusto
Leão,Sylvia Cardoso
Ferreira Neto,José Soares
author_role author
author2 Rodriguez,César Alejandro Rosales
Rocha,Vivianne Cambuí Mesquita
Ambrosio,Simone Rodriguez
Ohara,Patrícia Miyuki
Amaku,Marcos
Ferreira,Fernando
Dias,Ricardo Augusto
Leão,Sylvia Cardoso
Ferreira Neto,José Soares
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,Eugenia Márcia de Deus
Rodriguez,César Alejandro Rosales
Rocha,Vivianne Cambuí Mesquita
Ambrosio,Simone Rodriguez
Ohara,Patrícia Miyuki
Amaku,Marcos
Ferreira,Fernando
Dias,Ricardo Augusto
Leão,Sylvia Cardoso
Ferreira Neto,José Soares
dc.subject.por.fl_str_mv Mycobacterium avium
isolation
feces
swine
topic Mycobacterium avium
isolation
feces
swine
description Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000400019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.4 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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