Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex

Detalhes bibliográficos
Autor(a) principal: Spositto, F.l.e.
Data de Publicação: 2014
Outros Autores: Campanerut, P.a.z., Ghiraldi, L.d., Leite, C.q.f. [UNESP], Hirata, M.h., Hirata, R.d.c., Siqueira, V.l.d., Cardoso, R. Fressatti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1517-83822014000300012
http://hdl.handle.net/11449/114347
Resumo: We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
id UNSP_c43027ba477aac7715fe9e608f661e4c
oai_identifier_str oai:repositorio.unesp.br:11449/114347
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complexbovine tuberculosistuberculosisPCR-multiplexdiagnosisWe evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Estadual de Maringá Programa de Pós Graduação em Biociências e FisiopatologiaUniversidade Estadual de Maringá Departamento de Análises Clínicas e BiomedicinaUniversidade Estadual Paulista Faculdade de Ciências FarmacêuticasUniversidade de São Paulo Departamento de Análises Clínicas e Toxicológicas Faculdade de Ciências FarmacêuticasUniversidade Estadual Paulista Faculdade de Ciências FarmacêuticasSociedade Brasileira de MicrobiologiaUniversidade Estadual de Maringá (UEM)Universidade Estadual de Maringá (UEM)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Spositto, F.l.e.Campanerut, P.a.z.Ghiraldi, L.d.Leite, C.q.f. [UNESP]Hirata, M.h.Hirata, R.d.c.Siqueira, V.l.d.Cardoso, R. Fressatti2015-02-02T12:39:27Z2015-02-02T12:39:27Z2014-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article841-843application/pdfhttp://dx.doi.org/10.1590/S1517-83822014000300012Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014.1517-8382http://hdl.handle.net/11449/11434710.1590/S1517-83822014000300012S1517-83822014000300012S1517-83822014000300012.pdf2114570774349859SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Microbiology1.8100,630info:eu-repo/semantics/openAccess2024-06-24T13:07:52Zoai:repositorio.unesp.br:11449/114347Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:49:24.140096Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
spellingShingle Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
Spositto, F.l.e.
bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
title_short Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_fullStr Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_full_unstemmed Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
title_sort Multiplex-PCR for differentiation of Mycobacterium bovis from Mycobacterium tuberculosis complex
author Spositto, F.l.e.
author_facet Spositto, F.l.e.
Campanerut, P.a.z.
Ghiraldi, L.d.
Leite, C.q.f. [UNESP]
Hirata, M.h.
Hirata, R.d.c.
Siqueira, V.l.d.
Cardoso, R. Fressatti
author_role author
author2 Campanerut, P.a.z.
Ghiraldi, L.d.
Leite, C.q.f. [UNESP]
Hirata, M.h.
Hirata, R.d.c.
Siqueira, V.l.d.
Cardoso, R. Fressatti
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Maringá (UEM)
Universidade Estadual de Maringá (UEM)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Spositto, F.l.e.
Campanerut, P.a.z.
Ghiraldi, L.d.
Leite, C.q.f. [UNESP]
Hirata, M.h.
Hirata, R.d.c.
Siqueira, V.l.d.
Cardoso, R. Fressatti
dc.subject.por.fl_str_mv bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
topic bovine tuberculosis
tuberculosis
PCR-multiplex
diagnosis
description We evaluated a multiplex-PCR to differentiate Mycobacterium bovis from M. tuberculosis Complex (MTC) by one step amplification based on simultaneous detection of pncA 169C > G change in M. bovis and the IS6110 present in MTC species. Our findings showed the proposed multiplex-PCR is a very useful tool for complementation in differentiating M. bovis from other cultured MTC species.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
2015-02-02T12:39:27Z
2015-02-02T12:39:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1517-83822014000300012
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014.
1517-8382
http://hdl.handle.net/11449/114347
10.1590/S1517-83822014000300012
S1517-83822014000300012
S1517-83822014000300012.pdf
2114570774349859
url http://dx.doi.org/10.1590/S1517-83822014000300012
http://hdl.handle.net/11449/114347
identifier_str_mv Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 45, n. 3, p. 841-843, 2014.
1517-8382
10.1590/S1517-83822014000300012
S1517-83822014000300012
S1517-83822014000300012.pdf
2114570774349859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Microbiology
1.810
0,630
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 841-843
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129123728490496

Registros relacionados