Campylobacter jejuni in commercial eggs

Detalhes bibliográficos
Autor(a) principal: Fonseca,Belchiolina Beatriz
Data de Publicação: 2014
Outros Autores: Beletti,Marcelo Emílio, Melo,Roberta Torres de, Mendonça,Eliane Pereira, Coelho,Letícia Ríspoli, Nalevaiko,Priscila Christen, Rossi,Daise Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100011
Resumo: This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
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spelling Campylobacter jejuni in commercial eggscampylobacteriosiscommercial eggspenetrationThis study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.Sociedade Brasileira de Microbiologia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100011Brazilian Journal of Microbiology v.45 n.1 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000100011info:eu-repo/semantics/openAccessFonseca,Belchiolina BeatrizBeletti,Marcelo EmílioMelo,Roberta Torres deMendonça,Eliane PereiraCoelho,Letícia RíspoliNalevaiko,Priscila ChristenRossi,Daise Aparecidaeng2014-05-29T00:00:00Zoai:scielo:S1517-83822014000100011Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-05-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Campylobacter jejuni in commercial eggs
title Campylobacter jejuni in commercial eggs
spellingShingle Campylobacter jejuni in commercial eggs
Fonseca,Belchiolina Beatriz
campylobacteriosis
commercial eggs
penetration
title_short Campylobacter jejuni in commercial eggs
title_full Campylobacter jejuni in commercial eggs
title_fullStr Campylobacter jejuni in commercial eggs
title_full_unstemmed Campylobacter jejuni in commercial eggs
title_sort Campylobacter jejuni in commercial eggs
author Fonseca,Belchiolina Beatriz
author_facet Fonseca,Belchiolina Beatriz
Beletti,Marcelo Emílio
Melo,Roberta Torres de
Mendonça,Eliane Pereira
Coelho,Letícia Ríspoli
Nalevaiko,Priscila Christen
Rossi,Daise Aparecida
author_role author
author2 Beletti,Marcelo Emílio
Melo,Roberta Torres de
Mendonça,Eliane Pereira
Coelho,Letícia Ríspoli
Nalevaiko,Priscila Christen
Rossi,Daise Aparecida
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fonseca,Belchiolina Beatriz
Beletti,Marcelo Emílio
Melo,Roberta Torres de
Mendonça,Eliane Pereira
Coelho,Letícia Ríspoli
Nalevaiko,Priscila Christen
Rossi,Daise Aparecida
dc.subject.por.fl_str_mv campylobacteriosis
commercial eggs
penetration
topic campylobacteriosis
commercial eggs
penetration
description This study evaluated the ability of Campylobacter jejuni to penetrate through the pores of the shells of commercial eggs and colonize the interior of these eggs, which may become a risk factor for human infection. Furthermore, this study assessed the survival and viability of the bacteria in commercial eggs. The eggs were placed in contact with wood shavings infected with C. jejuni to check the passage of the bacteria. In parallel, the bacteria were inoculated directly into the air chamber to assess the viability in the egg yolk. To determine whether the albumen and egg fertility interferes with the entry and survival of bacteria, we used varying concentrations of albumen and SPF and commercial eggs. C. jejuni was recovered in SPF eggs (fertile) after three hours in contact with contaminated wood shavings but not in infertile commercial eggs. The colonies isolated in the SPF eggs were identified by multiplex PCR and the similarity between strains verified by RAPD-PCR. The bacteria grew in different concentrations of albumen in commercial and SPF eggs. We did not find C. jejuni in commercial eggs inoculated directly into the air chamber, but the bacteria were viable during all periods tested in the wood shavings. This study shows that consumption of commercial eggs infected with C. jejuni does not represent a potential risk to human health.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000100011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.1 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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