Viability and gene transcription of Campylobacter jejuni in infant milk formulas

Detalhes bibliográficos
Autor(a) principal: de Melo, Roberta Torres
Data de Publicação: 2019
Outros Autores: Pacheco, Carla Ribeiro, Monteiro, Guilherme Paz, Paiva Maia, Yara Cristina, Mendonça, Eliane Pereira, Braz, Raquelline Figueiredo, Valadares Júnior, Edson Campos, Rossi, Daise Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Bioscience journal (Online)
Texto Completo: https://seer.ufu.br/index.php/biosciencejournal/article/view/41901
Resumo: This study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription  of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations.
id UFU-14_980fadd0800c1ce775a55f00de4bba8d
oai_identifier_str oai:ojs.www.seer.ufu.br:article/41901
network_acronym_str UFU-14
network_name_str Bioscience journal (Online)
repository_id_str
spelling Viability and gene transcription of Campylobacter jejuni in infant milk formulasViabilidade e transcrição genética de Campylobacter jejuni em fórmulas lácteas infantisCampylobacteriosisInfantsGastroenteritis.Agricultural SciencesThis study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription  of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations.Este estudo simulou a contaminação de duas variedades de fórmulas de leite infantil com Campylobacter jejuni (caseiras e comerciais), que foram mantidas sob refrigeração (4-7ºC) por até 48 horas. O objetivo do estudo foi verificar a manutenção da viabilidade e capacidade de produzir transcritos de virulência e resistência a condições de estresse durante os períodos de 0 (após preparação), 24 e 48 horas. C. jejuni permaneceu viável durante todas as etapas analisadas e a presença de coliformes não foi detectada. Em geral, as contagens reduziram 1 ciclo log após 48 horas para todas as amostras, exceto o inóculo de 104 CFU/mL na fórmula comercial, que reduziu 2 ciclos logarítmicos, indicando maior lesão de C. jejuni nesta matriz alimentar. C. jejuni mostrou-se mais adaptado à matriz popular, devido à alta transcrição do gene relacionado à invasão celular, ciaB, e mais suscetível na matriz comercial, devido à alta transcrição de genes  relacionados a tolerância a condições de estresse (dnaJ, p19, sodB). A baixa dose infectante de C. jejuni, juntamente com maior vulnerabilidade de crianças menores de cinco anos, indicam a necessidade de cuidados na preparação e manutenção de fórmulas infantis, para prevenir o uso de matéria-prima contaminada e contaminação cruzada, especialmente em formulações caseiras.EDUFU2019-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4190110.14393/BJ-v35n5a2019-41901Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1504-1514Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1504-15141981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/41901/27075Brazil; ContemporaryCopyright (c) 2019 Roberta Torres de Melo, Carla Ribeiro Pacheco, Guilherme Paz Monteiro, Yara Cristina Paiva Maia, Eliane Pereira Mendonça, Raquelline Figueiredo Braz, Edson Campos Valadares Júnior, Daise Aparecida Rossihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessde Melo, Roberta TorresPacheco, Carla RibeiroMonteiro, Guilherme PazPaiva Maia, Yara CristinaMendonça, Eliane PereiraBraz, Raquelline Figueiredo Valadares Júnior, Edson CamposRossi, Daise Aparecida2022-01-26T03:57:02Zoai:ojs.www.seer.ufu.br:article/41901Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-26T03:57:02Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false
dc.title.none.fl_str_mv Viability and gene transcription of Campylobacter jejuni in infant milk formulas
Viabilidade e transcrição genética de Campylobacter jejuni em fórmulas lácteas infantis
title Viability and gene transcription of Campylobacter jejuni in infant milk formulas
spellingShingle Viability and gene transcription of Campylobacter jejuni in infant milk formulas
de Melo, Roberta Torres
Campylobacteriosis
Infants
Gastroenteritis.
Agricultural Sciences
title_short Viability and gene transcription of Campylobacter jejuni in infant milk formulas
title_full Viability and gene transcription of Campylobacter jejuni in infant milk formulas
title_fullStr Viability and gene transcription of Campylobacter jejuni in infant milk formulas
title_full_unstemmed Viability and gene transcription of Campylobacter jejuni in infant milk formulas
title_sort Viability and gene transcription of Campylobacter jejuni in infant milk formulas
author de Melo, Roberta Torres
author_facet de Melo, Roberta Torres
Pacheco, Carla Ribeiro
Monteiro, Guilherme Paz
Paiva Maia, Yara Cristina
Mendonça, Eliane Pereira
Braz, Raquelline Figueiredo
Valadares Júnior, Edson Campos
Rossi, Daise Aparecida
author_role author
author2 Pacheco, Carla Ribeiro
Monteiro, Guilherme Paz
Paiva Maia, Yara Cristina
Mendonça, Eliane Pereira
Braz, Raquelline Figueiredo
Valadares Júnior, Edson Campos
Rossi, Daise Aparecida
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv de Melo, Roberta Torres
Pacheco, Carla Ribeiro
Monteiro, Guilherme Paz
Paiva Maia, Yara Cristina
Mendonça, Eliane Pereira
Braz, Raquelline Figueiredo
Valadares Júnior, Edson Campos
Rossi, Daise Aparecida
dc.subject.por.fl_str_mv Campylobacteriosis
Infants
Gastroenteritis.
Agricultural Sciences
topic Campylobacteriosis
Infants
Gastroenteritis.
Agricultural Sciences
description This study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription  of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/41901
10.14393/BJ-v35n5a2019-41901
url https://seer.ufu.br/index.php/biosciencejournal/article/view/41901
identifier_str_mv 10.14393/BJ-v35n5a2019-41901
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://seer.ufu.br/index.php/biosciencejournal/article/view/41901/27075
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil; Contemporary
dc.publisher.none.fl_str_mv EDUFU
publisher.none.fl_str_mv EDUFU
dc.source.none.fl_str_mv Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1504-1514
Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1504-1514
1981-3163
reponame:Bioscience journal (Online)
instname:Universidade Federal de Uberlândia (UFU)
instacron:UFU
instname_str Universidade Federal de Uberlândia (UFU)
instacron_str UFU
institution UFU
reponame_str Bioscience journal (Online)
collection Bioscience journal (Online)
repository.name.fl_str_mv Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)
repository.mail.fl_str_mv biosciencej@ufu.br||
_version_ 1797069079651024896