Viability and gene transcription of Campylobacter jejuni in infant milk formulas
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Bioscience journal (Online) |
Texto Completo: | https://seer.ufu.br/index.php/biosciencejournal/article/view/41901 |
Resumo: | This study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations. |
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Viability and gene transcription of Campylobacter jejuni in infant milk formulasViabilidade e transcrição genética de Campylobacter jejuni em fórmulas lácteas infantisCampylobacteriosisInfantsGastroenteritis.Agricultural SciencesThis study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations.Este estudo simulou a contaminação de duas variedades de fórmulas de leite infantil com Campylobacter jejuni (caseiras e comerciais), que foram mantidas sob refrigeração (4-7ºC) por até 48 horas. O objetivo do estudo foi verificar a manutenção da viabilidade e capacidade de produzir transcritos de virulência e resistência a condições de estresse durante os períodos de 0 (após preparação), 24 e 48 horas. C. jejuni permaneceu viável durante todas as etapas analisadas e a presença de coliformes não foi detectada. Em geral, as contagens reduziram 1 ciclo log após 48 horas para todas as amostras, exceto o inóculo de 104 CFU/mL na fórmula comercial, que reduziu 2 ciclos logarítmicos, indicando maior lesão de C. jejuni nesta matriz alimentar. C. jejuni mostrou-se mais adaptado à matriz popular, devido à alta transcrição do gene relacionado à invasão celular, ciaB, e mais suscetível na matriz comercial, devido à alta transcrição de genes relacionados a tolerância a condições de estresse (dnaJ, p19, sodB). A baixa dose infectante de C. jejuni, juntamente com maior vulnerabilidade de crianças menores de cinco anos, indicam a necessidade de cuidados na preparação e manutenção de fórmulas infantis, para prevenir o uso de matéria-prima contaminada e contaminação cruzada, especialmente em formulações caseiras.EDUFU2019-10-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://seer.ufu.br/index.php/biosciencejournal/article/view/4190110.14393/BJ-v35n5a2019-41901Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1504-1514Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1504-15141981-3163reponame:Bioscience journal (Online)instname:Universidade Federal de Uberlândia (UFU)instacron:UFUenghttps://seer.ufu.br/index.php/biosciencejournal/article/view/41901/27075Brazil; ContemporaryCopyright (c) 2019 Roberta Torres de Melo, Carla Ribeiro Pacheco, Guilherme Paz Monteiro, Yara Cristina Paiva Maia, Eliane Pereira Mendonça, Raquelline Figueiredo Braz, Edson Campos Valadares Júnior, Daise Aparecida Rossihttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessde Melo, Roberta TorresPacheco, Carla RibeiroMonteiro, Guilherme PazPaiva Maia, Yara CristinaMendonça, Eliane PereiraBraz, Raquelline Figueiredo Valadares Júnior, Edson CamposRossi, Daise Aparecida2022-01-26T03:57:02Zoai:ojs.www.seer.ufu.br:article/41901Revistahttps://seer.ufu.br/index.php/biosciencejournalPUBhttps://seer.ufu.br/index.php/biosciencejournal/oaibiosciencej@ufu.br||1981-31631516-3725opendoar:2022-01-26T03:57:02Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU)false |
dc.title.none.fl_str_mv |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas Viabilidade e transcrição genética de Campylobacter jejuni em fórmulas lácteas infantis |
title |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
spellingShingle |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas de Melo, Roberta Torres Campylobacteriosis Infants Gastroenteritis. Agricultural Sciences |
title_short |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
title_full |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
title_fullStr |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
title_full_unstemmed |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
title_sort |
Viability and gene transcription of Campylobacter jejuni in infant milk formulas |
author |
de Melo, Roberta Torres |
author_facet |
de Melo, Roberta Torres Pacheco, Carla Ribeiro Monteiro, Guilherme Paz Paiva Maia, Yara Cristina Mendonça, Eliane Pereira Braz, Raquelline Figueiredo Valadares Júnior, Edson Campos Rossi, Daise Aparecida |
author_role |
author |
author2 |
Pacheco, Carla Ribeiro Monteiro, Guilherme Paz Paiva Maia, Yara Cristina Mendonça, Eliane Pereira Braz, Raquelline Figueiredo Valadares Júnior, Edson Campos Rossi, Daise Aparecida |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
de Melo, Roberta Torres Pacheco, Carla Ribeiro Monteiro, Guilherme Paz Paiva Maia, Yara Cristina Mendonça, Eliane Pereira Braz, Raquelline Figueiredo Valadares Júnior, Edson Campos Rossi, Daise Aparecida |
dc.subject.por.fl_str_mv |
Campylobacteriosis Infants Gastroenteritis. Agricultural Sciences |
topic |
Campylobacteriosis Infants Gastroenteritis. Agricultural Sciences |
description |
This study simulated the contamination of two varieties of infant milk formulas (homemade and commercial) with 103 and 104 CFU/mL of Campylobacter jejuni, that were kept under refrigeration (4-7ºC) for up to 48 hours. The aim of this study was to verify the maintenance of the viability and ability of Campylobacter jejuni to produce transcripts of virulence and resistance to stress conditions during periods of 0 (after preparation), 24 and 48 hours. C. jejuni remained viable during all analyzed stages and the presence of coliforms was not detected. In general, the counts reduced 1 log cycle after 48 hours for all samples, except the 104 CFU/mL inoculum of commercial formula, which reduced 2 log cycles, indicating greater injury of C. jejuni in this food matrix. C. jejuni showed to be more adapted to homemade matrix, due to high transcription of the gene related to cell invasion, ciaB, and more susceptible in the commercial matrix, due to the high transcription of genes related to conditions of stress tolerance (dnaJ, p19, sodB). The low infective dose of C. jejuni coupled with greater vulnerability of children less than five years indicate the need for care in the preparation and maintenance of infant formulas, to prevent the use of contaminated raw material and cross-contamination, especially in homemade formulations. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41901 10.14393/BJ-v35n5a2019-41901 |
url |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41901 |
identifier_str_mv |
10.14393/BJ-v35n5a2019-41901 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufu.br/index.php/biosciencejournal/article/view/41901/27075 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Brazil; Contemporary |
dc.publisher.none.fl_str_mv |
EDUFU |
publisher.none.fl_str_mv |
EDUFU |
dc.source.none.fl_str_mv |
Bioscience Journal ; Vol. 35 No. 5 (2019): Sept./Oct.; 1504-1514 Bioscience Journal ; v. 35 n. 5 (2019): Sept./Oct.; 1504-1514 1981-3163 reponame:Bioscience journal (Online) instname:Universidade Federal de Uberlândia (UFU) instacron:UFU |
instname_str |
Universidade Federal de Uberlândia (UFU) |
instacron_str |
UFU |
institution |
UFU |
reponame_str |
Bioscience journal (Online) |
collection |
Bioscience journal (Online) |
repository.name.fl_str_mv |
Bioscience journal (Online) - Universidade Federal de Uberlândia (UFU) |
repository.mail.fl_str_mv |
biosciencej@ufu.br|| |
_version_ |
1797069079651024896 |