Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii

Detalhes bibliográficos
Autor(a) principal: Zhu,Yanping
Data de Publicação: 2008
Outros Autores: Pan,Jieru, Qiu,Junzhi, Guan,Xiong
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022
Resumo: Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests.
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spelling Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecaniiChitinaseCloningOverexpressionHomology modelingVerticillium lecaniiEntomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests.Sociedade Brasileira de Microbiologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022Brazilian Journal of Microbiology v.39 n.2 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000200022info:eu-repo/semantics/openAccessZhu,YanpingPan,JieruQiu,JunzhiGuan,Xiongeng2008-07-31T00:00:00Zoai:scielo:S1517-83822008000200022Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-07-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
title Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
spellingShingle Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
Zhu,Yanping
Chitinase
Cloning
Overexpression
Homology modeling
Verticillium lecanii
title_short Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
title_full Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
title_fullStr Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
title_full_unstemmed Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
title_sort Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
author Zhu,Yanping
author_facet Zhu,Yanping
Pan,Jieru
Qiu,Junzhi
Guan,Xiong
author_role author
author2 Pan,Jieru
Qiu,Junzhi
Guan,Xiong
author2_role author
author
author
dc.contributor.author.fl_str_mv Zhu,Yanping
Pan,Jieru
Qiu,Junzhi
Guan,Xiong
dc.subject.por.fl_str_mv Chitinase
Cloning
Overexpression
Homology modeling
Verticillium lecanii
topic Chitinase
Cloning
Overexpression
Homology modeling
Verticillium lecanii
description Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests.
publishDate 2008
dc.date.none.fl_str_mv 2008-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822008000200022
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.39 n.2 2008
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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