Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022 |
Resumo: | Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests. |
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Brazilian Journal of Microbiology |
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Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecaniiChitinaseCloningOverexpressionHomology modelingVerticillium lecaniiEntomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests.Sociedade Brasileira de Microbiologia2008-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022Brazilian Journal of Microbiology v.39 n.2 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000200022info:eu-repo/semantics/openAccessZhu,YanpingPan,JieruQiu,JunzhiGuan,Xiongeng2008-07-31T00:00:00Zoai:scielo:S1517-83822008000200022Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-07-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
title |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
spellingShingle |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii Zhu,Yanping Chitinase Cloning Overexpression Homology modeling Verticillium lecanii |
title_short |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
title_full |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
title_fullStr |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
title_full_unstemmed |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
title_sort |
Isolation and characterization of a chitinase gene from entomopathogenic fungus Verticillium lecanii |
author |
Zhu,Yanping |
author_facet |
Zhu,Yanping Pan,Jieru Qiu,Junzhi Guan,Xiong |
author_role |
author |
author2 |
Pan,Jieru Qiu,Junzhi Guan,Xiong |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Zhu,Yanping Pan,Jieru Qiu,Junzhi Guan,Xiong |
dc.subject.por.fl_str_mv |
Chitinase Cloning Overexpression Homology modeling Verticillium lecanii |
topic |
Chitinase Cloning Overexpression Homology modeling Verticillium lecanii |
description |
Entomopathogenic fungus Verticillium lecanii is a promising whitefly and aphid control agent. Chitinases secreted by this insect pathogen have considerable importance in the biological control of some insect pests. An endochitinase gene Vlchit1 from the fungus was cloned and overexpressed in Escherichia coli. The Vlchit1 gene not only contains an open reading frame (ORF) which encodes a protein of 423 amino acids (aa), but also is interrupted by three short introns. A homology modelling of Vlchit1 protein showed that the chitinase Vlchit1 has a (α/β)8 TIM barrel structure. Overexpression test and Enzymatic activity assay indicated that the Vlchit1 is a functional enzyme that can hydrolyze the chitin substrate, so the Vlchit1 gene can service as a useful gene source for genetic manipulation leading to strain improvement of entomopathogenic fungi or constructing new transgenic plants with resistance to various fungal and insects pests. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200022 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000200022 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.2 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201770819584 |