Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus

Detalhes bibliográficos
Autor(a) principal: Flatschart,Roberto B.
Data de Publicação: 2000
Outros Autores: Resende,Maurício
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
Resumo: Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
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spelling Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virusAujeszky’s disease viruspolymerase chain reactiondot-blot hybridizationAcute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.Sociedade Brasileira de Microbiologia2000-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013Brazilian Journal of Microbiology v.31 n.4 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000400013info:eu-repo/semantics/openAccessFlatschart,Roberto B.Resende,Maurícioeng2001-05-23T00:00:00Zoai:scielo:S1517-83822000000400013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2001-05-23T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
spellingShingle Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
Flatschart,Roberto B.
Aujeszky’s disease virus
polymerase chain reaction
dot-blot hybridization
title_short Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_full Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_fullStr Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_full_unstemmed Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
title_sort Acute and latent infection in mice with a virulent strain of Aujeszky’s disease virus
author Flatschart,Roberto B.
author_facet Flatschart,Roberto B.
Resende,Maurício
author_role author
author2 Resende,Maurício
author2_role author
dc.contributor.author.fl_str_mv Flatschart,Roberto B.
Resende,Maurício
dc.subject.por.fl_str_mv Aujeszky’s disease virus
polymerase chain reaction
dot-blot hybridization
topic Aujeszky’s disease virus
polymerase chain reaction
dot-blot hybridization
description Acute and latent infections with the Brazilian LA031 strain of Aujeszky’s disease virus (ADV) were established in mice. Ultraviolet irradiated ADV administered subcutaneously was a successful way to establish latent infection. The presence of ADV was detected by PCR. Two sets of 22-mer primers were synthesized and used to amplify gG glycoprotein gene sequences in acute and latent infected trigeminal nerve ganglia. The specificity of the amplification was verified by dot-blot hybridization.
publishDate 2000
dc.date.none.fl_str_mv 2000-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400013
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822000000400013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.31 n.4 2000
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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