Development of DNA probe for detection of Aujeszky's disease virus

Detalhes bibliográficos
Autor(a) principal: Cândido,A.L.
Data de Publicação: 1999
Outros Autores: Barros,P.P., Resende,M., Flaschart,R.B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivo brasileiro de medicina veterinária e zootecnia (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001
Resumo: A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.
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spelling Development of DNA probe for detection of Aujeszky's disease virusAujeszky's disease virusdot-blot hybridizationradioactive probenon radioactive probeA DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.Universidade Federal de Minas Gerais, Escola de Veterinária1999-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.51 n.4 1999reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)instname:Universidade Federal de Minas Gerais (UFMG)instacron:UFMG10.1590/S0102-09351999000400001info:eu-repo/semantics/openAccessCândido,A.L.Barros,P.P.Resende,M.Flaschart,R.B.eng2001-04-17T00:00:00Zoai:scielo:S0102-09351999000400001Revistahttps://www.scielo.br/j/abmvz/PUBhttps://old.scielo.br/oai/scielo-oai.phpjournal@vet.ufmg.br||abmvz.artigo@abmvz.org.br1678-41620102-0935opendoar:2001-04-17T00:00Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)false
dc.title.none.fl_str_mv Development of DNA probe for detection of Aujeszky's disease virus
title Development of DNA probe for detection of Aujeszky's disease virus
spellingShingle Development of DNA probe for detection of Aujeszky's disease virus
Cândido,A.L.
Aujeszky's disease virus
dot-blot hybridization
radioactive probe
non radioactive probe
title_short Development of DNA probe for detection of Aujeszky's disease virus
title_full Development of DNA probe for detection of Aujeszky's disease virus
title_fullStr Development of DNA probe for detection of Aujeszky's disease virus
title_full_unstemmed Development of DNA probe for detection of Aujeszky's disease virus
title_sort Development of DNA probe for detection of Aujeszky's disease virus
author Cândido,A.L.
author_facet Cândido,A.L.
Barros,P.P.
Resende,M.
Flaschart,R.B.
author_role author
author2 Barros,P.P.
Resende,M.
Flaschart,R.B.
author2_role author
author
author
dc.contributor.author.fl_str_mv Cândido,A.L.
Barros,P.P.
Resende,M.
Flaschart,R.B.
dc.subject.por.fl_str_mv Aujeszky's disease virus
dot-blot hybridization
radioactive probe
non radioactive probe
topic Aujeszky's disease virus
dot-blot hybridization
radioactive probe
non radioactive probe
description A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.
publishDate 1999
dc.date.none.fl_str_mv 1999-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09351999000400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-09351999000400001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
publisher.none.fl_str_mv Universidade Federal de Minas Gerais, Escola de Veterinária
dc.source.none.fl_str_mv Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.51 n.4 1999
reponame:Arquivo brasileiro de medicina veterinária e zootecnia (Online)
instname:Universidade Federal de Minas Gerais (UFMG)
instacron:UFMG
instname_str Universidade Federal de Minas Gerais (UFMG)
instacron_str UFMG
institution UFMG
reponame_str Arquivo brasileiro de medicina veterinária e zootecnia (Online)
collection Arquivo brasileiro de medicina veterinária e zootecnia (Online)
repository.name.fl_str_mv Arquivo brasileiro de medicina veterinária e zootecnia (Online) - Universidade Federal de Minas Gerais (UFMG)
repository.mail.fl_str_mv journal@vet.ufmg.br||abmvz.artigo@abmvz.org.br
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