Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods

Detalhes bibliográficos
Autor(a) principal: Gomes,Bruna C.
Data de Publicação: 2007
Outros Autores: Esteves,Carolina T., Palazzo,Izabel C.V., Darini,Ana Lúcia C., Franco,Bernadette D.G.M., Martinis,Elaine C.P. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400007
Resumo: We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.
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spelling Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foodsEnterococcus spp.identificationAPI 20 STREPPCR multiplexfoodsWe evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.Sociedade Brasileira de Microbiologia2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400007Brazilian Journal of Microbiology v.38 n.4 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000400007info:eu-repo/semantics/openAccessGomes,Bruna C.Esteves,Carolina T.Palazzo,Izabel C.V.Darini,Ana Lúcia C.Franco,Bernadette D.G.M.Martinis,Elaine C.P. deeng2008-01-28T00:00:00Zoai:scielo:S1517-83822007000400007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-01-28T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
title Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
spellingShingle Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
Gomes,Bruna C.
Enterococcus spp.
identification
API 20 STREP
PCR multiplex
foods
title_short Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
title_full Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
title_fullStr Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
title_full_unstemmed Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
title_sort Correlation between API 20 STREP and multiplex PCR for identification of Enterococcus spp. isolated from Brazilian foods
author Gomes,Bruna C.
author_facet Gomes,Bruna C.
Esteves,Carolina T.
Palazzo,Izabel C.V.
Darini,Ana Lúcia C.
Franco,Bernadette D.G.M.
Martinis,Elaine C.P. de
author_role author
author2 Esteves,Carolina T.
Palazzo,Izabel C.V.
Darini,Ana Lúcia C.
Franco,Bernadette D.G.M.
Martinis,Elaine C.P. de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes,Bruna C.
Esteves,Carolina T.
Palazzo,Izabel C.V.
Darini,Ana Lúcia C.
Franco,Bernadette D.G.M.
Martinis,Elaine C.P. de
dc.subject.por.fl_str_mv Enterococcus spp.
identification
API 20 STREP
PCR multiplex
foods
topic Enterococcus spp.
identification
API 20 STREP
PCR multiplex
foods
description We evaluated the suitability of API 20 STREP and multiplex PCR to speciate 52 Enterococcus spp. obtained from Brazilian foods. A high percentage of isolates (78.9%) presented discrepant results between evaluated tests. Similar results were obtained for six E. faecalis and five E. faecium. The PCR multiplex was more effective than API 20 STREP for complete identification of the isolates.
publishDate 2007
dc.date.none.fl_str_mv 2007-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.4 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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