Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic

Detalhes bibliográficos
Autor(a) principal: Shentu,Xuping
Data de Publicação: 2014
Outros Autores: Zhan,Xiaohuan, Ma,Zheng, Yu,Xiaoping, Zhang,Chuanxi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100036
Resumo: The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.
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spelling Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlicTrichoderma brevicompactumgarlicendophytic fungusTrichoderminantifungal activityThe endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.Sociedade Brasileira de Microbiologia2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100036Brazilian Journal of Microbiology v.45 n.1 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014005000036info:eu-repo/semantics/openAccessShentu,XupingZhan,XiaohuanMa,ZhengYu,XiaopingZhang,Chuanxieng2014-05-29T00:00:00Zoai:scielo:S1517-83822014000100036Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-05-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
title Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
spellingShingle Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
Shentu,Xuping
Trichoderma brevicompactum
garlic
endophytic fungus
Trichodermin
antifungal activity
title_short Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
title_full Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
title_fullStr Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
title_full_unstemmed Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
title_sort Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic
author Shentu,Xuping
author_facet Shentu,Xuping
Zhan,Xiaohuan
Ma,Zheng
Yu,Xiaoping
Zhang,Chuanxi
author_role author
author2 Zhan,Xiaohuan
Ma,Zheng
Yu,Xiaoping
Zhang,Chuanxi
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Shentu,Xuping
Zhan,Xiaohuan
Ma,Zheng
Yu,Xiaoping
Zhang,Chuanxi
dc.subject.por.fl_str_mv Trichoderma brevicompactum
garlic
endophytic fungus
Trichodermin
antifungal activity
topic Trichoderma brevicompactum
garlic
endophytic fungus
Trichodermin
antifungal activity
description The endophytic fungus strain 0248, isolated from garlic, was identified as Trichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 µgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 µgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin against Colletotrichum lindemuthianum (EC50 = 25.60 µgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100036
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000100036
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014005000036
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.1 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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