Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis

Detalhes bibliográficos
Autor(a) principal: Oliveira,Fabiana da Rocha
Data de Publicação: 2017
Outros Autores: Noronha,Maria das Dores Nogueira, Lozano,Jorge Luis Lopez
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000300365
Resumo: Abstract INTRODUCTION: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. METHODS: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. RESULTS AND CONCLUSIONS: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice.
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spelling Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensisMicrurus surinamensisNeurotoxinsPhospholipase A2Biological activitiesAbstract INTRODUCTION: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. METHODS: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. RESULTS AND CONCLUSIONS: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice.Sociedade Brasileira de Medicina Tropical - SBMT2017-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000300365Revista da Sociedade Brasileira de Medicina Tropical v.50 n.3 2017reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/0037-8682-0408-2016info:eu-repo/semantics/openAccessOliveira,Fabiana da RochaNoronha,Maria das Dores NogueiraLozano,Jorge Luis Lopezeng2017-09-12T00:00:00Zoai:scielo:S0037-86822017000300365Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2017-09-12T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
spellingShingle Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
Oliveira,Fabiana da Rocha
Micrurus surinamensis
Neurotoxins
Phospholipase A2
Biological activities
title_short Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_full Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_fullStr Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_full_unstemmed Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
title_sort Biological and molecular properties of yellow venom of the Amazonian coral snake Micrurus surinamensis
author Oliveira,Fabiana da Rocha
author_facet Oliveira,Fabiana da Rocha
Noronha,Maria das Dores Nogueira
Lozano,Jorge Luis Lopez
author_role author
author2 Noronha,Maria das Dores Nogueira
Lozano,Jorge Luis Lopez
author2_role author
author
dc.contributor.author.fl_str_mv Oliveira,Fabiana da Rocha
Noronha,Maria das Dores Nogueira
Lozano,Jorge Luis Lopez
dc.subject.por.fl_str_mv Micrurus surinamensis
Neurotoxins
Phospholipase A2
Biological activities
topic Micrurus surinamensis
Neurotoxins
Phospholipase A2
Biological activities
description Abstract INTRODUCTION: The coral snake Micrurus surinamensis, which is widely distributed throughout Amazonia, has a neurotoxic venom. It is important to characterize the biological and molecular properties of this venom in order to develop effective antitoxins. METHODS: Toxins from the venom of M. surinamensis were analyzed by two-dimensional polyacrylamide gel electrophoresis and their neurotoxic effects in vivo were evaluated. RESULTS AND CONCLUSIONS: Most proteins in the venom had masses < 14kDa, low phospholipase A2 activity, and no proteolytic activity. The toxins inhibited the coagulation cascade. The venom had neurotoxic effects in mice, with a median lethal dose upon intravenous administration of 700 µg/kg. Immunogenic studies revealed abundant cross-reactivity of antielapidic serum with 14kDa toxins and limited cross-reactivity with toxins < 10kDa. These results indicate that antielapidic serum against M. surinamensis venom has weak potency (0.35mg/ml) in mice.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000300365
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000300365
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0037-8682-0408-2016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.50 n.3 2017
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
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reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
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