Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007 |
Resumo: | INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. |
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Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental modelTrypanosoma cruziMexicoBiological characterizationGenetic characterizationINTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI.Sociedade Brasileira de Medicina Tropical - SBMT2011-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007Revista da Sociedade Brasileira de Medicina Tropical v.44 n.6 2011reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822011005000058info:eu-repo/semantics/openAccessGómez-Hernández,CésarRezende-Oliveira,KarineNascentes,Gabriel Antônio NogueiraBatista,Lara RochaKappel,Henrique BorgesMartinez-Ibarra,José AlejandroTrujillo Contreras,FranciscoLages-Silva,ElianeRamírez,Luis Eduardoeng2012-01-06T00:00:00Zoai:scielo:S0037-86822011000600007Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2012-01-06T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
spellingShingle |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model Gómez-Hernández,César Trypanosoma cruzi Mexico Biological characterization Genetic characterization |
title_short |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_full |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_fullStr |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_full_unstemmed |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
title_sort |
Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model |
author |
Gómez-Hernández,César |
author_facet |
Gómez-Hernández,César Rezende-Oliveira,Karine Nascentes,Gabriel Antônio Nogueira Batista,Lara Rocha Kappel,Henrique Borges Martinez-Ibarra,José Alejandro Trujillo Contreras,Francisco Lages-Silva,Eliane Ramírez,Luis Eduardo |
author_role |
author |
author2 |
Rezende-Oliveira,Karine Nascentes,Gabriel Antônio Nogueira Batista,Lara Rocha Kappel,Henrique Borges Martinez-Ibarra,José Alejandro Trujillo Contreras,Francisco Lages-Silva,Eliane Ramírez,Luis Eduardo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Gómez-Hernández,César Rezende-Oliveira,Karine Nascentes,Gabriel Antônio Nogueira Batista,Lara Rocha Kappel,Henrique Borges Martinez-Ibarra,José Alejandro Trujillo Contreras,Francisco Lages-Silva,Eliane Ramírez,Luis Eduardo |
dc.subject.por.fl_str_mv |
Trypanosoma cruzi Mexico Biological characterization Genetic characterization |
topic |
Trypanosoma cruzi Mexico Biological characterization Genetic characterization |
description |
INTRODUCTION: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. METHODS: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD - (random amplified polymorphic DNA). RESULTS: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10(6) to 10(7) parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. CONCLUSIONS: The Mexican T. cruzi strains are myotrophic and belong to group TcI. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000600007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822011005000058 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.44 n.6 2011 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122157288128512 |