Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus

Detalhes bibliográficos
Autor(a) principal: Reiter,Keli Cristine
Data de Publicação: 2012
Outros Autores: Sambrano,Gustavo Enck, Villa,Bárbara, Paim,Thiago Galvão da Silva, Oliveira,Caio Fernando de, d'Azevedo,Pedro Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011
Resumo: INTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. METHODS: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. RESULTS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). CONCLUSIONS: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin.
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spelling Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureusStaphylococcus aureusRifampicinVancomycinBiofilmResistanceINTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. METHODS: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. RESULTS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). CONCLUSIONS: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin.Sociedade Brasileira de Medicina Tropical - SBMT2012-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011Revista da Sociedade Brasileira de Medicina Tropical v.45 n.4 2012reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822012000400011info:eu-repo/semantics/openAccessReiter,Keli CristineSambrano,Gustavo EnckVilla,BárbaraPaim,Thiago Galvão da SilvaOliveira,Caio Fernando ded'Azevedo,Pedro Alveseng2012-08-23T00:00:00Zoai:scielo:S0037-86822012000400011Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2012-08-23T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
title Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
spellingShingle Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
Reiter,Keli Cristine
Staphylococcus aureus
Rifampicin
Vancomycin
Biofilm
Resistance
title_short Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
title_full Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
title_fullStr Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
title_full_unstemmed Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
title_sort Rifampicin fails to eradicate mature biofilm formed by methicillin-resistant Staphylococcus aureus
author Reiter,Keli Cristine
author_facet Reiter,Keli Cristine
Sambrano,Gustavo Enck
Villa,Bárbara
Paim,Thiago Galvão da Silva
Oliveira,Caio Fernando de
d'Azevedo,Pedro Alves
author_role author
author2 Sambrano,Gustavo Enck
Villa,Bárbara
Paim,Thiago Galvão da Silva
Oliveira,Caio Fernando de
d'Azevedo,Pedro Alves
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Reiter,Keli Cristine
Sambrano,Gustavo Enck
Villa,Bárbara
Paim,Thiago Galvão da Silva
Oliveira,Caio Fernando de
d'Azevedo,Pedro Alves
dc.subject.por.fl_str_mv Staphylococcus aureus
Rifampicin
Vancomycin
Biofilm
Resistance
topic Staphylococcus aureus
Rifampicin
Vancomycin
Biofilm
Resistance
description INTRODUCTION: Antimicrobial activity on biofilms depends on their molecular size, positive charges, permeability coefficient, and bactericidal activity. Vancomycin is the primary choice for methicillin-resistant Staphylococcus aureus (MRSA) infection treatment; rifampicin has interesting antibiofilm properties, but its effectivity remains poorly defined. METHODS: Rifampicin activity alone and in combination with vancomycin against biofilm-forming MRSA was investigated, using a twofold serial broth microtiter method, biofilm challenge, and bacterial count recovery. RESULTS: Minimal inhibitory concentration (MIC) and minimal bactericidal concentration for vancomycin and rifampicin ranged from 0.5 to 1mg/l and 0.008 to 4mg/l, and from 1 to 4mg/l and 0.06 to 32mg/l, respectively. Mature biofilms were submitted to rifampicin and vancomycin exposure, and minimum biofilm eradication concentration ranged from 64 to 32,000 folds and from 32 to 512 folds higher than those for planktonic cells, respectively. Vancomycin (15mg/l) in combination with rifampicin at 6 dilutions higher each isolate MIC did not reach in vitro biofilm eradication but showed biofilm inhibitory capacity (1.43 and 0.56log10 CFU/ml reduction for weak and strong biofilm producers, respectively; p<0.05). CONCLUSIONS: In our setting, rifampicin alone failed to effectively kill biofilm-forming MRSA, demonstrating stronger inability to eradicate mature biofilm compared with vancomycin.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000400011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86822012000400011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.45 n.4 2012
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
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instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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