Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays
Autor(a) principal: | |
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Data de Publicação: | 1997 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000400005 |
Resumo: | The influence of time and temperature on the storage of an alkaline antigen of L.major-like and L.(V.) braziliensis promastigotes added or not of a proteases inhibitor (PMSF) was evaluted by means of an IgG-ELISA. Antibodies in assays using L. major-like antigen stored at -20oC for 6 monsths had a statistically lower geometric mean titer (GMT) and different 95% confidence interval limits (CL) than antigens stored otherwise, as assessed by the "t" statistic. The PMSF L. major-like antigen after storage for 6 months at a temperature of 4oC had the same GMT and 95% CL displayed at time zero as well as when storage for 4 and 6 months at -20oC. Significant diferences were not found when L.(V.) braziliensis antigens were stored at times and temperatures mentioned; the PMSF antigen stored for 2 months at -70oC resulted in a lower serum GMT and 95% CL than any other, as assessed by the "t" statistic. Antigen performance did not show any statistical difference associated to the addition of PMSF within the same species; the largest difference between antigens was that between PMSF-L. (V.) braziliensis and L. major-like without PMSF. |
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Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassaysLeishmania major-likeLeishmania (V.) braziliensisProteases inhibitor (PMSF)IgG-ELISAGeometric mean titerThe influence of time and temperature on the storage of an alkaline antigen of L.major-like and L.(V.) braziliensis promastigotes added or not of a proteases inhibitor (PMSF) was evaluted by means of an IgG-ELISA. Antibodies in assays using L. major-like antigen stored at -20oC for 6 monsths had a statistically lower geometric mean titer (GMT) and different 95% confidence interval limits (CL) than antigens stored otherwise, as assessed by the "t" statistic. The PMSF L. major-like antigen after storage for 6 months at a temperature of 4oC had the same GMT and 95% CL displayed at time zero as well as when storage for 4 and 6 months at -20oC. Significant diferences were not found when L.(V.) braziliensis antigens were stored at times and temperatures mentioned; the PMSF antigen stored for 2 months at -70oC resulted in a lower serum GMT and 95% CL than any other, as assessed by the "t" statistic. Antigen performance did not show any statistical difference associated to the addition of PMSF within the same species; the largest difference between antigens was that between PMSF-L. (V.) braziliensis and L. major-like without PMSF.Sociedade Brasileira de Medicina Tropical - SBMT1997-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000400005Revista da Sociedade Brasileira de Medicina Tropical v.30 n.4 1997reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86821997000400005info:eu-repo/semantics/openAccessCeleste,Beatriz JulietaGuimarães,Maria Carolina SoaresSouza,José Maria Pacheco deeng2000-06-14T00:00:00Zoai:scielo:S0037-86821997000400005Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2000-06-14T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
title |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
spellingShingle |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays Celeste,Beatriz Julieta Leishmania major-like Leishmania (V.) braziliensis Proteases inhibitor (PMSF) IgG-ELISA Geometric mean titer |
title_short |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
title_full |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
title_fullStr |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
title_full_unstemmed |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
title_sort |
Evaluation of Leishmania antigens preparation and storage for use in enzyme immunoassays |
author |
Celeste,Beatriz Julieta |
author_facet |
Celeste,Beatriz Julieta Guimarães,Maria Carolina Soares Souza,José Maria Pacheco de |
author_role |
author |
author2 |
Guimarães,Maria Carolina Soares Souza,José Maria Pacheco de |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Celeste,Beatriz Julieta Guimarães,Maria Carolina Soares Souza,José Maria Pacheco de |
dc.subject.por.fl_str_mv |
Leishmania major-like Leishmania (V.) braziliensis Proteases inhibitor (PMSF) IgG-ELISA Geometric mean titer |
topic |
Leishmania major-like Leishmania (V.) braziliensis Proteases inhibitor (PMSF) IgG-ELISA Geometric mean titer |
description |
The influence of time and temperature on the storage of an alkaline antigen of L.major-like and L.(V.) braziliensis promastigotes added or not of a proteases inhibitor (PMSF) was evaluted by means of an IgG-ELISA. Antibodies in assays using L. major-like antigen stored at -20oC for 6 monsths had a statistically lower geometric mean titer (GMT) and different 95% confidence interval limits (CL) than antigens stored otherwise, as assessed by the "t" statistic. The PMSF L. major-like antigen after storage for 6 months at a temperature of 4oC had the same GMT and 95% CL displayed at time zero as well as when storage for 4 and 6 months at -20oC. Significant diferences were not found when L.(V.) braziliensis antigens were stored at times and temperatures mentioned; the PMSF antigen stored for 2 months at -70oC resulted in a lower serum GMT and 95% CL than any other, as assessed by the "t" statistic. Antigen performance did not show any statistical difference associated to the addition of PMSF within the same species; the largest difference between antigens was that between PMSF-L. (V.) braziliensis and L. major-like without PMSF. |
publishDate |
1997 |
dc.date.none.fl_str_mv |
1997-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86821997000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.30 n.4 1997 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
_version_ |
1752122150285737984 |