Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista da Sociedade Brasileira de Medicina Tropical |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001 |
Resumo: | In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis. |
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Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodateParacoccidioidomycosisAntigensMetaperiodateDiagnosisIn this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.Sociedade Brasileira de Medicina Tropical - SBMT2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001Revista da Sociedade Brasileira de Medicina Tropical v.41 n.4 2008reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822008000400001info:eu-repo/semantics/openAccessFerreira,Ana PaulaCôrrea,ThadeuCunha,RosângelaMarques,Marcos JoséMontesano,Maria AngelaSouza,Maria AparecidaTeixeira,Henrique Coutoeng2008-10-03T00:00:00Zoai:scielo:S0037-86822008000400001Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2008-10-03T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false |
dc.title.none.fl_str_mv |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
title |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
spellingShingle |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate Ferreira,Ana Paula Paracoccidioidomycosis Antigens Metaperiodate Diagnosis |
title_short |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
title_full |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
title_fullStr |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
title_full_unstemmed |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
title_sort |
Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate |
author |
Ferreira,Ana Paula |
author_facet |
Ferreira,Ana Paula Côrrea,Thadeu Cunha,Rosângela Marques,Marcos José Montesano,Maria Angela Souza,Maria Aparecida Teixeira,Henrique Couto |
author_role |
author |
author2 |
Côrrea,Thadeu Cunha,Rosângela Marques,Marcos José Montesano,Maria Angela Souza,Maria Aparecida Teixeira,Henrique Couto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Ferreira,Ana Paula Côrrea,Thadeu Cunha,Rosângela Marques,Marcos José Montesano,Maria Angela Souza,Maria Aparecida Teixeira,Henrique Couto |
dc.subject.por.fl_str_mv |
Paracoccidioidomycosis Antigens Metaperiodate Diagnosis |
topic |
Paracoccidioidomycosis Antigens Metaperiodate Diagnosis |
description |
In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0037-86822008000400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
publisher.none.fl_str_mv |
Sociedade Brasileira de Medicina Tropical - SBMT |
dc.source.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical v.41 n.4 2008 reponame:Revista da Sociedade Brasileira de Medicina Tropical instname:Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
instname_str |
Sociedade Brasileira de Medicina Tropical (SBMT) |
instacron_str |
SBMT |
institution |
SBMT |
reponame_str |
Revista da Sociedade Brasileira de Medicina Tropical |
collection |
Revista da Sociedade Brasileira de Medicina Tropical |
repository.name.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT) |
repository.mail.fl_str_mv |
||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br |
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1752122154447536128 |