Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate

Detalhes bibliográficos
Autor(a) principal: Ferreira,Ana Paula
Data de Publicação: 2008
Outros Autores: Côrrea,Thadeu, Cunha,Rosângela, Marques,Marcos José, Montesano,Maria Angela, Souza,Maria Aparecida, Teixeira,Henrique Couto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Sociedade Brasileira de Medicina Tropical
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001
Resumo: In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.
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spelling Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodateParacoccidioidomycosisAntigensMetaperiodateDiagnosisIn this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.Sociedade Brasileira de Medicina Tropical - SBMT2008-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001Revista da Sociedade Brasileira de Medicina Tropical v.41 n.4 2008reponame:Revista da Sociedade Brasileira de Medicina Tropicalinstname:Sociedade Brasileira de Medicina Tropical (SBMT)instacron:SBMT10.1590/S0037-86822008000400001info:eu-repo/semantics/openAccessFerreira,Ana PaulaCôrrea,ThadeuCunha,RosângelaMarques,Marcos JoséMontesano,Maria AngelaSouza,Maria AparecidaTeixeira,Henrique Coutoeng2008-10-03T00:00:00Zoai:scielo:S0037-86822008000400001Revistahttps://www.sbmt.org.br/portal/revista/ONGhttps://old.scielo.br/oai/scielo-oai.php||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br1678-98490037-8682opendoar:2008-10-03T00:00Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)false
dc.title.none.fl_str_mv Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
title Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
spellingShingle Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
Ferreira,Ana Paula
Paracoccidioidomycosis
Antigens
Metaperiodate
Diagnosis
title_short Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
title_full Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
title_fullStr Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
title_full_unstemmed Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
title_sort Human serum antibody reactivity towards Paracoccidioides brasiliensis antigens treated with sodium metaperiodate
author Ferreira,Ana Paula
author_facet Ferreira,Ana Paula
Côrrea,Thadeu
Cunha,Rosângela
Marques,Marcos José
Montesano,Maria Angela
Souza,Maria Aparecida
Teixeira,Henrique Couto
author_role author
author2 Côrrea,Thadeu
Cunha,Rosângela
Marques,Marcos José
Montesano,Maria Angela
Souza,Maria Aparecida
Teixeira,Henrique Couto
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira,Ana Paula
Côrrea,Thadeu
Cunha,Rosângela
Marques,Marcos José
Montesano,Maria Angela
Souza,Maria Aparecida
Teixeira,Henrique Couto
dc.subject.por.fl_str_mv Paracoccidioidomycosis
Antigens
Metaperiodate
Diagnosis
topic Paracoccidioidomycosis
Antigens
Metaperiodate
Diagnosis
description In this study, we evaluated the profile of anti-Paracoccidioides brasiliensis immunoglobulin isotypes in serum from patients with the acute and chronic forms of paracoccidioidomycosis, using the whole Paracoccidioides brasiliensis antigen and the antigen treated with sodium metaperiodate. All the immunoglobulin isotypes present in the serum from patients with the acute and chronic forms of paracoccidioidomycosis presented higher reactivity towards the whole antigen than to the antigen treated with metaperiodate (P < 0.05). The reactivity of IgG and IgM to the antigen treated with metaperiodate was greater in serum from patients with the acute form of the disease (P < 0.05), while IgA was more reactive in serum from patients with the chronic form (P < 0.05). There was greater reactivity of IgG1 and IgG2 to the whole antigen and the antigen treated with metaperiodate in the serum from patients with paracoccidioidomycosis than there was in serum from patients with other parasitic infections (P < 0.05). Furthermore, IgG1 from patients with the acute form recognized the 19kDa, 27kDa and 31kDa antigens in the western blot test. Thus, the results suggest that modifications to the epitopes of Paracoccidioides brasiliensis antigens may help to improve the immunodiagnosis of paracoccidioidomycosis.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822008000400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0037-86822008000400001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
publisher.none.fl_str_mv Sociedade Brasileira de Medicina Tropical - SBMT
dc.source.none.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical v.41 n.4 2008
reponame:Revista da Sociedade Brasileira de Medicina Tropical
instname:Sociedade Brasileira de Medicina Tropical (SBMT)
instacron:SBMT
instname_str Sociedade Brasileira de Medicina Tropical (SBMT)
instacron_str SBMT
institution SBMT
reponame_str Revista da Sociedade Brasileira de Medicina Tropical
collection Revista da Sociedade Brasileira de Medicina Tropical
repository.name.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical - Sociedade Brasileira de Medicina Tropical (SBMT)
repository.mail.fl_str_mv ||dalmo@rsbmt.uftm.edu.br|| rsbmt@rsbmt.uftm.edu.br
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