Isolation and characterization of partially purified leptospiral antigens

Detalhes bibliográficos
Autor(a) principal: Koury, M.C.
Data de Publicação: 1991
Outros Autores: Rangel, H.A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28859
Resumo: The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.
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spelling Isolation and characterization of partially purified leptospiral antigens Isolamento e caracterização de antígenos parcialmente purificados de Leptospira LeptospiraAntigensPurificationDiagnosis The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction. Extrato metílico de Leptospira interrogans sorovar canicola foi purificado por precipitação com acetona ou acetona e clorofórmio. A antigenicidade não foi alterada por aquecimento ou tratamento com pepsina e pronase, entretanto foi perdida quando o antígeno foi tratado com ácido periódico. Análise química revelou a presença de 40% de carboidrato (22% de metilpentose, 28% de hexose), 4% de proteína, 20% de lípide e 2,7% de fosfato. Reação de fixação de complemento realizada com soros de pacientes com leptospirose apresentou concordância com a reação de aglutinação microscópica. Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1991-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28859Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 5 (1991); 391-396 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 5 (1991); 391-396 Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 5 (1991); 391-396 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28859/30712Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessKoury, M.C.Rangel, H.A.2012-07-02T01:31:08Zoai:revistas.usp.br:article/28859Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:37.554016Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Isolation and characterization of partially purified leptospiral antigens
Isolamento e caracterização de antígenos parcialmente purificados de Leptospira
title Isolation and characterization of partially purified leptospiral antigens
spellingShingle Isolation and characterization of partially purified leptospiral antigens
Koury, M.C.
Leptospira
Antigens
Purification
Diagnosis
title_short Isolation and characterization of partially purified leptospiral antigens
title_full Isolation and characterization of partially purified leptospiral antigens
title_fullStr Isolation and characterization of partially purified leptospiral antigens
title_full_unstemmed Isolation and characterization of partially purified leptospiral antigens
title_sort Isolation and characterization of partially purified leptospiral antigens
author Koury, M.C.
author_facet Koury, M.C.
Rangel, H.A.
author_role author
author2 Rangel, H.A.
author2_role author
dc.contributor.author.fl_str_mv Koury, M.C.
Rangel, H.A.
dc.subject.por.fl_str_mv Leptospira
Antigens
Purification
Diagnosis
topic Leptospira
Antigens
Purification
Diagnosis
description The methanol extract of Leptospira interrogans serovar canicola was purified by precipitation with acetone or acetone and chloroform. The antigenicity of the antigen was not altered by heating or treatment with pepsin and pronase. However the antigenicity was lost when the antigen was treated with periodic acid. Chemical analysis revealed the presence of 40% carbohydrate (22% methylpentose, 28%; hexoses),4% protein, 20% lipid and 2,7% phosphate. The complement fixation test with sera from patients with leptospirosis agreed with the microscopic agglutination reaction.
publishDate 1991
dc.date.none.fl_str_mv 1991-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28859
url https://www.revistas.usp.br/rimtsp/article/view/28859
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28859/30712
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 No. 5 (1991); 391-396
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 33 Núm. 5 (1991); 391-396
Revista do Instituto de Medicina Tropical de São Paulo; v. 33 n. 5 (1991); 391-396
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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