Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/44 |
Resumo: | The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g).In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity found |
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Chemical characterization and antibacterial action of brown propolis extract from Southern BrazilCaracterização química e ação antibacteriana de extrato de própolis marrom da região sul do BrasilAntimicrobiano, produto natural, tempo de açãoAntimicrobial, natural products, action time.The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g).In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity foundO objetivo desse estudo foi caracterizar quimicamente uma amostra de própolis marrom e determinar o tempo de ação necessário para eliminar micro-organismos causadores de mastite bovina. Foi preparado extrato hidroalcoólico de própolis marrom (25 mg/mL) e esse foi avaliado quanto ao teor de compostos fenólicos totais, flavonóides totais, análise qualitativa e quantitativa dos compostos fenólicos por cromatografia líquida acoplada a espectrometria de massas e atividade antibacteriana frente a Escherichia coli, Staphylococcus spp, Streptococcus spp. Foram encontrados 34,39% e 13,46% de compostos fenólicos e flavonóides, respectivamente, dentre os quais se destacam ácido ferúlico (0,09 mg/g), ácido cafeico (0,17 mg/g) e ácido p-cumárico (3,39 mg/g). Nos primeiros 15 minutos de incubação todas as contagens de unidades formadoras de colônias (UFC) diferiram das contagens iniciais (p<0,01). Em 15 minutos de exposição ao extrato, Escherichia coli foi inibida em 38,72% e diferiu dos demais gêneros (p<0,01, n=6), sendo que Staphylococcus spp teve inibição de 46,03% e Streptococcus spp de 50,8%. Após 2 horas de incubação, o gênero Streptococcus foi totalmente eliminado, Staphylococcus spp após 3 horas e E. coli após 4 horas. Notadamente, E. coli foi a bactéria mais resistente, seguida de S. aureus. As demais espécies de Staphylococcus e Streptococcus não apresentaram diferenças entre as suas contagens. Conclui-se que a composição da amostra de própolis marrom do sul do Brasil apresenta grandes teores de ácidos fenólicos com ação antibacteriana comprovada, o que explica a atividade bactericida encontrada.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2016-12-16info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/44Brazilian Journal of Veterinary Medicine; Vol. 38 No. 4 (2016); 365-371Revista Brasileira de Medicina Veterinária; v. 38 n. 4 (2016); 365-3712527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/44/33Picoli, TonyPeter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares Zani, João Luiz Vargas, Gilberto D’ÁvilaHubner, SilviaFischer, Gefersoninfo:eu-repo/semantics/openAccess2020-12-23T17:25:35Zoai:ojs.rbmv.org:article/44Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:25:35Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil Caracterização química e ação antibacteriana de extrato de própolis marrom da região sul do Brasil |
title |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
spellingShingle |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil Picoli, Tony Antimicrobiano, produto natural, tempo de ação Antimicrobial, natural products, action time. |
title_short |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_full |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_fullStr |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_full_unstemmed |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
title_sort |
Chemical characterization and antibacterial action of brown propolis extract from Southern Brazil |
author |
Picoli, Tony |
author_facet |
Picoli, Tony Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares Zani, João Luiz Vargas, Gilberto D’Ávila Hubner, Silvia Fischer, Geferson |
author_role |
author |
author2 |
Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares Zani, João Luiz Vargas, Gilberto D’Ávila Hubner, Silvia Fischer, Geferson |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Picoli, Tony Peter, Cristina Mendes Hoffmann, Jessica Fernanda Latosinski, Giulia Soares Zani, João Luiz Vargas, Gilberto D’Ávila Hubner, Silvia Fischer, Geferson |
dc.subject.por.fl_str_mv |
Antimicrobiano, produto natural, tempo de ação Antimicrobial, natural products, action time. |
topic |
Antimicrobiano, produto natural, tempo de ação Antimicrobial, natural products, action time. |
description |
The aim of this study was to characterize chemically a sample of brown propolis and determine the time of action needed for eliminating microorganisms causing bovine mastitis. It was prepared hydroalcoholic extract of brown propolis (25 mg/mL) and this was evaluated for content of total phenolic compounds, total flavonoids, qualitative and quantitative analysis of phenolic compounds by liquid chromatography coupled to mass spectrometry and antibacterial activity against Escherichia coli, Staphylococcus spp, Streptococcus spp. It was found 34.39% and 13.46% of flavonoids and phenolic compounds, respectively, among which stand out ferulic acid (0.09 mg/g), caffeic acid (0.17 mg/g) and p-coumaric acid (3.39 mg/g).In the first 15 minutes incubation, all counts differed from the initial counts (p<0.01). With 15 minutes of exposure to the extract, Escherichia coli was inhibited by 38.72% and differed from the other bacterial genus (p<0.01, n=6). Staphylococcus spp. has had inhibition of 46.03% and Streptotococcus spp. of 50.8%. After 2 hours incubation, the genus Streptococcus was totally eliminated, Staphylococcus spp. after 3 hours and E. coli after 4 hours. Clearly, E. coli was more resistant bacteria than S. aureus. The other species of Staphylococcus and Streptococcus did not show large differences between the counts. We concluded that the composition of the brown propolis sample from Southern Brazil showed large levels of phenolic acids with proven antibacterial action, what explains the bactericidal activity found |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-16 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/44 |
url |
https://rbmv.org/BJVM/article/view/44 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/44/33 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 38 No. 4 (2016); 365-371 Revista Brasileira de Medicina Veterinária; v. 38 n. 4 (2016); 365-371 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313106313576448 |