A manual method to obtain platelet rich plasma

Detalhes bibliográficos
Autor(a) principal: Marques,Fabiana Paulino
Data de Publicação: 2014
Outros Autores: Ingham,Sheila Jean McNeill, Forgas,Andrea, Franciozi,Carlos Eduardo da Silveira, Sasaki,Pedro Henrique, Abdalla,Rene Jorge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Ortopédica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522014000200075
Resumo: OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.
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spelling A manual method to obtain platelet rich plasmaPlatelet-rich plasmaBlood plateletsMethods.OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.ATHA EDITORA2014-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522014000200075Acta Ortopédica Brasileira v.22 n.2 2014reponame:Acta Ortopédica Brasileira (Online)instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)instacron:SBOT10.1590/1413-78522014220200798info:eu-repo/semantics/openAccessMarques,Fabiana PaulinoIngham,Sheila Jean McNeillForgas,AndreaFranciozi,Carlos Eduardo da SilveiraSasaki,Pedro HenriqueAbdalla,Rene Jorgeeng2015-10-26T00:00:00Zoai:scielo:S1413-78522014000200075Revistahttp://www.actaortopedica.com.br/https://old.scielo.br/oai/scielo-oai.php1atha@uol.com.br||actaortopedicabrasileira@uol.com.br1809-44061413-7852opendoar:2015-10-26T00:00Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)false
dc.title.none.fl_str_mv A manual method to obtain platelet rich plasma
title A manual method to obtain platelet rich plasma
spellingShingle A manual method to obtain platelet rich plasma
Marques,Fabiana Paulino
Platelet-rich plasma
Blood platelets
Methods.
title_short A manual method to obtain platelet rich plasma
title_full A manual method to obtain platelet rich plasma
title_fullStr A manual method to obtain platelet rich plasma
title_full_unstemmed A manual method to obtain platelet rich plasma
title_sort A manual method to obtain platelet rich plasma
author Marques,Fabiana Paulino
author_facet Marques,Fabiana Paulino
Ingham,Sheila Jean McNeill
Forgas,Andrea
Franciozi,Carlos Eduardo da Silveira
Sasaki,Pedro Henrique
Abdalla,Rene Jorge
author_role author
author2 Ingham,Sheila Jean McNeill
Forgas,Andrea
Franciozi,Carlos Eduardo da Silveira
Sasaki,Pedro Henrique
Abdalla,Rene Jorge
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marques,Fabiana Paulino
Ingham,Sheila Jean McNeill
Forgas,Andrea
Franciozi,Carlos Eduardo da Silveira
Sasaki,Pedro Henrique
Abdalla,Rene Jorge
dc.subject.por.fl_str_mv Platelet-rich plasma
Blood platelets
Methods.
topic Platelet-rich plasma
Blood platelets
Methods.
description OBJECTIVE: This study is to report a manual method to obtain platelet rich plasma (PRP).METHODS: For this study 61 ml of peripheral blood was obtained and submitted to centrifugation at 541g for 5 min. The centrifugation separates the blood into three components: red blood cells, buffy coat and platelet rich plasma. Blood and platelet rich plasma samples were sent to the Hospital's Laboratory and platelets and leukocytes were measured.RESULTS: A sample of 637 blood donors was evaluated. The platelet yield efficiency was 86.77% and the increase in platelet concentration factor was 2.89 times. The increase in leukocyte concentration factor was 1.97 times.CONCLUSION: The method described here produces leukocyte-rich and platelet-rich plasma with a high platelet and leukocyte increased factor. Level of Evidence IV, Controlled Laboratory Study.
publishDate 2014
dc.date.none.fl_str_mv 2014-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522014000200075
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-78522014000200075
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1413-78522014220200798
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ATHA EDITORA
publisher.none.fl_str_mv ATHA EDITORA
dc.source.none.fl_str_mv Acta Ortopédica Brasileira v.22 n.2 2014
reponame:Acta Ortopédica Brasileira (Online)
instname:Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron:SBOT
instname_str Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
instacron_str SBOT
institution SBOT
reponame_str Acta Ortopédica Brasileira (Online)
collection Acta Ortopédica Brasileira (Online)
repository.name.fl_str_mv Acta Ortopédica Brasileira (Online) - Sociedade Brasileira de Ortopedia e Traumatologia (SBOT)
repository.mail.fl_str_mv 1atha@uol.com.br||actaortopedicabrasileira@uol.com.br
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